Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/29

From 2010.igem.org

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(New page: {{:Team:Tokyo_Metropolitan/Header}} ==2010/09/29 Wednesday (Naoto)== ===Experiment:Electrophoresis of insert check PCR=== '''member''' naoto and bambi75 '''material''' *PCR production ...)
 
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{{:Team:Tokyo_Metropolitan/Header}}
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{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
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__NOTOC__
==2010/09/29 Wednesday (Naoto)==
==2010/09/29 Wednesday (Naoto)==
-
===Experiment:Electrophoresis of insert check PCR===
+
===Experiment:Insert check PCR===
'''member'''
'''member'''
-
naoto and bambi75
+
naoto and NEX
'''material'''
'''material'''
-
*PCR production (bcsD(''A.xylinum'') )
+
*bcsC,D(''A.xylinum'') in colonies of '''E.coli'''
-
If you want to know other materials,refer to protocol4
+
If you want to know other materials,refer to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR protocol3]
'''procedure'''
'''procedure'''
-
see protocol4
+
see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR protocol3]
-
'''result'''
+
===Experiment:PCR of PT7-RBS-bcsA~D(''A.xylinum''),PT7-RBS-E~G(E.coli),bcsQ(''E.coli''),RBS-T7polymerase===
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Bands didn't appear at all
+
-
 
+
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===Experiment:Electrophoresis of bcsEFG,Q===
+
'''member'''
'''member'''
-
naoto and bambi75
+
naoto and NEX
'''material'''
'''material'''
-
*PCR production (bcsEFG,Q(''E.coli'') )
+
*A colony of ''A.xylinum''(for templete DNA)
-
If you want to know other materials,refer to protocol4
+
*bcsE~G(E.coli)
 +
*bcsQ(''E.coli'')
 +
*T7polymerase(BBa_K145001)
 +
If you want to know other materials,refer to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR protocol3]
'''procedure'''
'''procedure'''
-
see protocol4
+
see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR protocol3]
-
'''result'''
+
===Experiment:digestion of bcsB(''E.coli'')===
-
Although many bands appeared,bands of bcsEFG and bcsQ also appeared
+
-
 
+
-
===Experiment:Electrophoresis of insert check PCR===
+
'''member'''
'''member'''
-
naoto and bambi75
+
naoto and easily
'''material'''
'''material'''
-
*PCR production (bcsD(''A.xylinum'') )
+
*bcsB(''E.coli'')  
-
If you want to know other materials,refer to protocol4
+
If you want to know other materials,refer to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol6:DNA_Purification_with_silica_gel protocol6]
'''procedure'''
'''procedure'''
-
see protocol4
+
follow to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol6:DNA_Purification_with_silica_gel protocol6]
-
===Experiment:Purification bcsA(''E.coli'')===
+
===Experiment:subculture of ''A.xylinum''===
'''member'''
'''member'''
-
naoto and bambi75
+
naoto and easily
'''material'''
'''material'''
-
*bcsQ,EFG(''E.coli'') in agarose gel
+
see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol1:Grow_up_a_culture_of_A.xylinum protocol1]
-
If you want to know other materials,refer to protocol5
+
A.xylinum(subcultured at 9/1)
'''procedure'''
'''procedure'''
-
follow to protocol5
+
see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol1:Grow_up_a_culture_of_A.xylinum protocol1]
-
===Experiment:digestion of bcsEFG,Q(''E.coli'')===
+
===Experiment:Transformation===
'''member'''
'''member'''
-
naoto and bambi75
+
naoto and easily
'''material'''
'''material'''
-
*bcsQ,EFG(''E.coli'')  
+
*parts of bcsA,B(''A.xylinum'')  
-
If you want to know other materials,refer to protocol6
+
If you want to know other materials,refer to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol8:Ligation protocol8]
'''procedure'''
'''procedure'''
-
follow to protocol6
+
see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol8:Ligation protocol8]
-
===Experiment:Electrophoresis of bcsEFG,Q===
+
===Experiment:Electrophoresis of insert check PCR===
'''member'''
'''member'''
-
naoto and bambi75
+
naoto  
'''material'''
'''material'''
-
*PCR production (bcsEFG,Q(''E.coli'') )
+
*PCR production (bcsC,D(''A.xylinum'') )
-
If you want to know other materials,refer to protocol4
+
If you want to know other materials,refer to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol4:Agarose_gel_electrophoresis protocol4]
'''procedure'''
'''procedure'''
-
see protocol4
+
see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol4:Agarose_gel_electrophoresis protocol4]
'''result'''
'''result'''
-
Both bands of bcsEFG and bcsQ disappeared
 
-
===Experiment:Transformation===
+
Bands(102 and 104) of bcsD(''A.xylinum'') could be seen slightly
 +
 
 +
So we prepared precultures for miniprep
 +
 
 +
===Experiment:Electrophoresis of PT7-RBS-bcsA~D(''A.xylinum''),PT7-RBS-E~G(''E.coli'')===
'''member'''
'''member'''
-
naoto and bambi75
+
naoto  
'''material'''
'''material'''
-
*parts of bcsA,B,C,D(''A.xylinum'') and bcsA(''E.coli'')
+
*PCR production (PT7-RBS-bcsA~D(''A.xylinum''),PT7-RBS-E~G(''E.coli''))
-
If you want to know other materials,refer to protocol8
+
If you want to know other materials,refer to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol4:Agarose_gel_electrophoresis protocol4]
'''procedure'''
'''procedure'''
-
see protocol8
+
see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol4:Agarose_gel_electrophoresis protocol4]
 +
 
 +
'''result'''
 +
 
 +
All bands don't have collect length.

Latest revision as of 16:05, 27 October 2010


E.coli Fiber Project Notebook

August 2010
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2010/09/29 Wednesday (Naoto)

Experiment:Insert check PCR

member

naoto and NEX

material

  • bcsC,D(A.xylinum) in colonies of E.coli

If you want to know other materials,refer to protocol3

procedure

see protocol3

Experiment:PCR of PT7-RBS-bcsA~D(A.xylinum),PT7-RBS-E~G(E.coli),bcsQ(E.coli),RBS-T7polymerase

member

naoto and NEX

material

  • A colony of A.xylinum(for templete DNA)
  • bcsE~G(E.coli)
  • bcsQ(E.coli)
  • T7polymerase(BBa_K145001)

If you want to know other materials,refer to protocol3

procedure

see protocol3

Experiment:digestion of bcsB(E.coli)

member

naoto and easily

material

  • bcsB(E.coli)

If you want to know other materials,refer to protocol6

procedure

follow to protocol6

Experiment:subculture of A.xylinum

member

naoto and easily

material

see protocol1 A.xylinum(subcultured at 9/1)

procedure

see protocol1

Experiment:Transformation

member

naoto and easily

material

  • parts of bcsA,B(A.xylinum)

If you want to know other materials,refer to protocol8

procedure

see protocol8

Experiment:Electrophoresis of insert check PCR

member

naoto

material

  • PCR production (bcsC,D(A.xylinum) )

If you want to know other materials,refer to protocol4

procedure

see protocol4

result

Bands(102 and 104) of bcsD(A.xylinum) could be seen slightly

So we prepared precultures for miniprep

Experiment:Electrophoresis of PT7-RBS-bcsA~D(A.xylinum),PT7-RBS-E~G(E.coli)

member

naoto

material

  • PCR production (PT7-RBS-bcsA~D(A.xylinum),PT7-RBS-E~G(E.coli))

If you want to know other materials,refer to protocol4

procedure

see protocol4

result

All bands don't have collect length.
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