Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/16

From 2010.igem.org

(Difference between revisions)

Latest revision as of 15:36, 27 October 2010

E.coli Fiber Project Notebook

2010/09/16 Thuesday (Bambi75)

Experiment:PCR

Member

naoto and bambi75

Materials,Procedure

Same protocol4

95℃3min
96℃1min
51℃1min
72℃3min
10℃∞

（96℃～72℃/30cycle）

Experiment:Agarose gel Electrophoresis

Member

Same above

Materials,Procedure

Same protocol4

PCR production (pSB1C3)

Preparation:Make Agarose Gel Electrophoresis

Member

Same above

Materials and Procedure

Same protocol4

Preparation:Medium making

Member

Same above

Materials and Procedure

Same protocol1

Experiment:Ligation

Member

Same above

Materials and Procedure

follow protocol8

bcsA,B,C and D
pSB1C3

@@ Line 1: / Line 1: @@
 {{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
+__NOTOC__
 ==2010/09/16 Thuesday (Bambi75)==
 ===Experiment:PCR===
-'''member'''
+:'''Member'''
-naoto and bambi75
+:naoto and bambi75
-'''materials,Procedure'''
+:'''Materials,Procedure'''
-Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
+:Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
-*95℃3min
+:*95℃3min
-*96℃1min
+:*96℃1min
-*51℃1min
+:*51℃1min
-*72℃3min
+:*72℃3min
-*10℃∞
+:*10℃∞
-（96℃～72℃/30cycle）
+:（96℃～72℃/30cycle）
-===Experiment:Agarose Gels Electro-phoresis===
+===Experiment:Agarose gel Electrophoresis===
-'''member'''
+:'''Member'''
-Same above
+:Same above
-'''Materials,Procedure'''
+:'''Materials,Procedure'''
-Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
+:Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
-PCR Product of insert is pBS1C3
+:*PCR production (pSB1C3)
-===Experiment:Experiment:Make Agarose Gels Electro-phoresis===
+===Preparation:Make Agarose Gel Electrophoresis===
-'''Member'''
+:'''Member'''
-Same above
+:Same above
-'''Materials and Procedure'''
+:'''Materials and Procedure'''
-Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
+:Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
 ===Preparation:Medium making===
-'''member'''
+:'''Member'''
-Same above
+:Same above
-'''materials and procedure'''
+:'''Materials and Procedure'''
-Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol1</a></html>
+:Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol1</a></html>
 ===Experiment:Ligation===
-'''member'''
+:'''Member'''
-Same above
-'''materials and procedure'''
-follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol8</a></html>
-*bcsA,B and C(E.coli).
-*bcsA,B,C and D(A.xylium)
-*pSB1C3
+:Same above
+:'''Materials and Procedure'''
+:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol8</a></html>
+:*bcsA,B,C and D
+:*pSB1C3
 <br/>