Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/16

From 2010.igem.org

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{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
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__NOTOC__
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==2010/09/16 Thuesday (Bambi75)==
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{{:Team:Tokyo_Metropolitan/Header}}
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===Experiment:PCR===
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:'''Member'''
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:naoto and bambi75
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:'''Materials,Procedure'''
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==PCR/pBS1C3==
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:Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
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:*95℃3min
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:*96℃1min
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:*51℃1min
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:*72℃3min
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:*10℃∞
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:(96℃~72℃/30cycle)
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===member===
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===Experiment:Agarose gel Electrophoresis===
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naoto and bambi75
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-
===materials,Procedure===
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:'''Member'''
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Same on August 23th.
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-
*95℃3min
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-
*96℃1min
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*51℃1min
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*72℃3min
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-
*10℃∞
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-
(96℃~72℃/30cycle)
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-
==Agarose Gels Electro-phoresis==
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:Same above
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===member===
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:'''Materials,Procedure'''
-
Same above
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-
===Materials,Procedure===
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-
Same protocol on August 16th.
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-
PCR Product of insert is pBS1C3
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-
==Experiment:Make Agarose Gels Electro-phoresis==
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:Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
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===Member===
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:*PCR production (pSB1C3)
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Same above
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-
===Materials and Procedure===
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-
Same protocol on August 16th.
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-
==Medium making==
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===member===
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===Preparation:Make Agarose Gel Electrophoresis===
-
Same above
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:'''Member'''
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===materials and procedure===
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:Same above
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Same protocol on August 10th.
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 +
:'''Materials and Procedure'''
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==Ligation==
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:Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
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===member===
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===Preparation:Medium making===
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Same above
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-
===materials and procedure===
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:'''Member'''
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follow the protocol(Ligation).
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*bcsA,B and C(E.coli).
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:Same above
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*bcsA,B,C and D(A.xylium)
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*pSB1C3
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:'''Materials and Procedure'''
 +
 
 +
:Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol1</a></html>
 +
 
 +
===Experiment:Ligation===
 +
 
 +
:'''Member'''
 +
 
 +
:Same above
 +
 
 +
:'''Materials and Procedure'''
 +
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol8</a></html>
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:*bcsA,B,C and D
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:*pSB1C3
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 +
<br/>

Latest revision as of 15:36, 27 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
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September 2010
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19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
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31


2010/09/16 Thuesday (Bambi75)

Experiment:PCR

Member
naoto and bambi75
Materials,Procedure
Same protocol4
  • 95℃3min
  • 96℃1min
  • 51℃1min
  • 72℃3min
  • 10℃∞
(96℃~72℃/30cycle)

Experiment:Agarose gel Electrophoresis

Member
Same above
Materials,Procedure
Same protocol4
  • PCR production (pSB1C3)

Preparation:Make Agarose Gel Electrophoresis

Member
Same above
Materials and Procedure
Same protocol4

Preparation:Medium making

Member
Same above
Materials and Procedure
Same protocol1

Experiment:Ligation

Member
Same above
Materials and Procedure
follow protocol8
  • bcsA,B,C and D
  • pSB1C3