Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/16

From 2010.igem.org

(Difference between revisions)

Latest revision as of 15:36, 27 October 2010

E.coli Fiber Project Notebook

2010/09/16 Thuesday (Bambi75)

Experiment:PCR

Member

naoto and bambi75

Materials,Procedure

Same protocol4

95℃3min
96℃1min
51℃1min
72℃3min
10℃∞

（96℃～72℃/30cycle）

Experiment:Agarose gel Electrophoresis

Member

Same above

Materials,Procedure

Same protocol4

PCR production (pSB1C3)

Preparation:Make Agarose Gel Electrophoresis

Member

Same above

Materials and Procedure

Same protocol4

Preparation:Medium making

Member

Same above

Materials and Procedure

Same protocol1

Experiment:Ligation

Member

Same above

Materials and Procedure

follow protocol8

bcsA,B,C and D
pSB1C3

@@ Line 1: / Line 1: @@
+{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
+__NOTOC__
+==2010/09/16 Thuesday (Bambi75)==
-{{:Team:Tokyo_Metropolitan/Header}}
+===Experiment:PCR===
+:'''Member'''
+:naoto and bambi75
+:'''Materials,Procedure'''
-==PCR/pBS1C3==
+:Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
+:*95℃3min
+:*96℃1min
+:*51℃1min
+:*72℃3min
+:*10℃∞
+:（96℃～72℃/30cycle）
-===member===
+===Experiment:Agarose gel Electrophoresis===
-naoto and bambi75
-===materials,Procedure===
+:'''Member'''
-Same on August 23th.
-*95℃3min
-*96℃1min
-*51℃1min
-*72℃3min
-*10℃∞
-（96℃～72℃/30cycle）
-==Agarose Gels Electro-phoresis==
+:Same above
-===member===
+:'''Materials,Procedure'''
-Same above
-===Materials,Procedure===
-Same protocol on August 16th.
-PCR Product of insert is pBS1C3
-==Experiment:Make Agarose Gels Electro-phoresis==
+:Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
-===Member===
+:*PCR production (pSB1C3)
-Same above
-===Materials and Procedure===
-Same protocol on August 16th.
-==Medium making==
-===member===
+===Preparation:Make Agarose Gel Electrophoresis===
-Same above
+:'''Member'''
-===materials and procedure===
+:Same above
-Same protocol on August 10th.
+:'''Materials and Procedure'''
-==Ligation==
+:Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
-===member===
+===Preparation:Medium making===
-Same above
-===materials and procedure===
+:'''Member'''
-follow the protocol(Ligation).
+:Same above
+:'''Materials and Procedure'''
+:Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol1</a></html>
+===Experiment:Ligation===
+:'''Member'''
+:Same above
+:'''Materials and Procedure'''
+:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol8</a></html>
+:*bcsA,B,C and D
+:*pSB1C3
+<br/>