Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/25

From 2010.igem.org

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===Experiment:PCR===
===Experiment:PCR===
-
'''Member'''<br/>
+
:'''Member'''<br/>
-
easily
+
:easily
-
'''Material'''<br/>
+
:'''Material'''<br/>
-
*sense primers of pSB1C3
+
:*templete DNA:pSB1C3
-
*antisense primers of pSB1C3
+
:*sense primers of pSB1C3
-
 
+
:*antisense primers of pSB1C3
-
'''Procedure'''<br/>
+
-
Refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR">protocol3</a></html>
+
 +
:'''Procedure'''<br/>
 +
:Refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR">protocol3</a></html>
===Experiment:Ligatiom===
===Experiment:Ligatiom===
-
'''Member'''<br/>
+
:'''Member'''<br/>
-
easily   
+
:easily   
-
'''Material'''<br/>
+
:'''Material'''<br/>
-
*bcsA from E.coli 20μl
+
:*bcsA from E.coli 20μl
-
*bcsB from E.coli 20μl
+
:*bcsB from E.coli 20μl
-
*2×ligation mix  40μl
+
:*2×ligation mix  40μl
-
 
+
-
'''Procedure'''<br/>
+
-
Follow to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol7:Ligation">protocol7</a></html>
+
 +
:'''Procedure'''<br/>
 +
:Follow to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol7:Ligation">protocol7</a></html>
===Experiment:Agarose gel electrophoresis===
===Experiment:Agarose gel electrophoresis===
-
'''Member'''<br />
+
:'''Member'''<br />
-
easily
+
:easily
-
'''Material'''
+
:'''Material'''
-
*Today's PCR productions
+
:*Today's PCR productions
-
*Today's ligation productions
+
:*Today's ligation productions
-
*Agarose gel
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:*Agarose gel
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'''Procedure'''<br/>
+
:'''Procedure'''<br/>
-
Refer to <html><a href="Agarose gel electrophoresis">protocol4</a></html>
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:Refer to <html><a href="Agarose gel electrophoresis">protocol4</a></html>
-
'''Result'''<br/>
+
:'''Result'''<br/>
[[Image:100925.jpg]]<br/>
[[Image:100925.jpg]]<br/>
-
pSB1C3 were appeared
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:pSB1C3 were appeared
===Experiment:DNA purification with silica gel===
===Experiment:DNA purification with silica gel===
-
'''Member'''<br />
+
:'''Member'''<br />
-
easily
+
:easily
-
'''Materials'''<br />
+
:'''Materials'''<br />
-
*pSB1C3
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:*pSB1C3
-
'''Procedure'''<br />
+
:'''Procedure'''<br />
-
See <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol5-2:DNA_Purification_with_silica_gel">protocol5-2</a></html>
+
:See <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol6:DNA_Purification_with_silica_gel">protocol6</a></html>
 +
===Experiment:Ligatiom===
 +
:'''Member'''<br/>
 +
:easily 
 +
:'''Material'''<br/>
 +
:*pSB1C3 3μl
 +
:*bcsA,B,C,D from A.xylinum or bcsA from E.coli 3μl
 +
:*2×ligation mix  6μl
-
===Experiment:Ligatiom===
+
:'''Procedure'''<br/>
-
'''Member'''<br/>
+
:Follow to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol7:Ligation">protocol7</a></html>
-
easily 
+
-
 
+
-
'''Material'''<br/>
+
-
*pSB1C3 3μl
+
-
*bcsA,B,C,D from A.xylinum or bcsA from E.coli 3μl
+
-
*2×ligation mix  6μl
+
-
'''Procedure'''<br/>
+
<br/>
-
Follow to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol7:Ligation">protocol7</a></html>
+

Latest revision as of 14:57, 27 October 2010


E.coli Fiber Project Notebook

August 2010
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September 2010
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October 2010
SUNMONTUEWEDTHUFRISAT
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2010/09/25 Saturday(NEX)

Experiment:PCR

Member
easily
Material
  • templete DNA:pSB1C3
  • sense primers of pSB1C3
  • antisense primers of pSB1C3
Procedure
Refer to protocol3

Experiment:Ligatiom

Member
easily
Material
  • bcsA from E.coli 20μl
  • bcsB from E.coli 20μl
  • 2×ligation mix 40μl
Procedure
Follow to protocol7

Experiment:Agarose gel electrophoresis

Member
easily
Material
  • Today's PCR productions
  • Today's ligation productions
  • Agarose gel
Procedure
Refer to protocol4
Result

100925.jpg

pSB1C3 were appeared

Experiment:DNA purification with silica gel

Member
easily
Materials
  • pSB1C3
Procedure
See protocol6

Experiment:Ligatiom

Member
easily
Material
  • pSB1C3 3μl
  • bcsA,B,C,D from A.xylinum or bcsA from E.coli 3μl
  • 2×ligation mix 6μl
Procedure
Follow to protocol7

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