Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/23

From 2010.igem.org

(Difference between revisions)
(Experiment:Insert check Electrophoresis)
(Experiment:Electrophoresis)
 
(3 intermediate revisions not shown)
Line 65: Line 65:
:'''Materials and Procedure'''
:'''Materials and Procedure'''
:*Colony PCR production
:*Colony PCR production
-
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">the protocol(Electrophoresis)</a></html>
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">protocol4</a></html>
:'''Result'''
:'''Result'''
Line 77: Line 77:
:'''Materials and Procedure'''
:'''Materials and Procedure'''
-
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Transformation">the protocol(Transformation)</a></html>
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Transformation">protocol9</a></html>
===Experiment:Electrophoresis===
===Experiment:Electrophoresis===
Line 85: Line 85:
:'''Materials and Procedure'''
:'''Materials and Procedure'''
-
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">the protocol(Electrophoresis)</a></html>
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">protocol4</a></html>
:'''Result'''
:'''Result'''

Latest revision as of 14:27, 27 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

2010/9/23 Thursday (watachin)

Experiment:PCR

Member
naoto
Materials and Procedure
E.coli introduced bcsA,B,C,D
follow protocol3

Experiment:Plating

Member
watachin
Materials and Procedure
plated E.coli(JM109) that transformated bcs genes(A.xlinum) to LB + cam medium from LB broth

Experiment:Colony PCR

Member
naoto
Materials and Procedure
Colony PCR production of bcs genes
follow protocol3

Experiment:Insert check Electrophoresis

Member
watachin
Materials and Procedure
follow protocol5
Result

2010-09-23-01.jpg 2010-09-23-02.jpg

Experiment:Electrophoresis of bcs genes

Member
naoto and watachin
Materials and Procedure
follow the protocol(Electrophoresis)

Experiment:DNA Extraction from agarose gel

Member
naoto and watachin
Materials and Procedure
follow protocol5

Experiment:Electrophoresis

Member
naoto and watachin
Materials and Procedure
  • Colony PCR production
follow protocol4
Result

2010-09-23-03.jpg

Experiment:Transformation

Member
watachin
Materials and Procedure
follow protocol9

Experiment:Electrophoresis

Member
naoto and watachin
Materials and Procedure
follow protocol4
Result

2010-09-23-04.jpg

Experiment:DNA Extraction from agarose gel

Member
naoto and watachin
Materials and Procedure
follow protocol5