Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/23

From 2010.igem.org

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{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
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__NOTOC__
==2010/9/23 Thursday (watachin)==
==2010/9/23 Thursday (watachin)==
===Experiment:PCR===
===Experiment:PCR===
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'''Member'''<br />
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:'''Member'''<br />
-
naoto
+
:naoto
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'''Materials and Procedure'''
+
:'''Materials and Procedure'''
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Direct PCR of bcs genes
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:E.coli introduced bcsA,B,C,D
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+
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follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">the protocol(Direct PCR)</a></html>
+
 +
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">protocol3</a></html>
===Experiment:Plating===
===Experiment:Plating===
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'''Member'''<br />
+
:'''Member'''<br />
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watachin
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:watachin
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'''Materials and Procedure'''
+
:'''Materials and Procedure'''
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plated E.coli(JM109) that transformated bcs genes(A.xlinum) to LB + cam medium from LB broth
+
:plated E.coli(JM109) that transformated bcs genes(A.xlinum) to LB + cam medium from LB broth
 +
===Experiment:Colony PCR===
 +
:'''Member'''<br />
 +
:naoto
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===Experiment:Insert check PCR===
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:'''Materials and Procedure'''
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'''Member'''<br />
+
-
naoto
+
-
'''Materials and Procedure'''
+
:Colony PCR production of bcs genes
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Insert check of bcs genes
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">protocol3</a></html>
-
 
+
-
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">the protocol(Direct PCR)</a></html>
+
===Experiment:Insert check Electrophoresis===
===Experiment:Insert check Electrophoresis===
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'''Member'''<br />
+
:'''Member'''<br />
-
watachin
+
:watachin
-
'''Materials and Procedure'''
+
:'''Materials and Procedure'''
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follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">the protocol(Electrophoresis)</a></html>
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">protocol5</a></html>
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'''Result'''
+
:'''Result'''
[[Image:2010-09-23-01.jpg]]
[[Image:2010-09-23-01.jpg]]
Line 46: Line 44:
===Experiment:Electrophoresis of bcs genes===
===Experiment:Electrophoresis of bcs genes===
-
'''Member'''<br />
+
:'''Member'''<br />
-
naoto and watachin
+
:naoto and watachin
-
'''Materials and Procedure'''
+
:'''Materials and Procedure'''
-
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">the protocol(Electrophoresis)</a></html>
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">the protocol(Electrophoresis)</a></html>
-
'''Result'''
+
===Experiment:DNA Extraction from agarose gel===
 +
:'''Member'''<br />
 +
:naoto and watachin
 +
:'''Materials and Procedure'''
-
===Experiment:DNA purification from agarose gel with QIAGEN===
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">protocol5</a></html>
-
'''Member'''<br />
+
-
naoto and watachin
+
-
'''Materials and Procedure'''
+
===Experiment:Electrophoresis===
-
 
+
:'''Member'''<br />
-
 
+
:naoto and watachin
-
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">the protocol(DNA purification from agarose gel with QIAGEN)</a></html>
+
-
 
+
-
 
+
-
===Experiment:Insert check Electrophoresis===
+
-
'''Member'''<br />
+
-
naoto and watachin
+
-
 
+
-
'''Materials and Procedure'''
+
-
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">the protocol(Electrophoresis)</a></html>
+
:'''Materials and Procedure'''
 +
:*Colony PCR production
 +
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">protocol4</a></html>
-
'''Result'''
+
:'''Result'''
[[Image:2010-09-23-03.jpg]]
[[Image:2010-09-23-03.jpg]]
===Experiment:Transformation===
===Experiment:Transformation===
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'''Member'''<br />
+
:'''Member'''<br />
-
watachin
+
:watachin
-
'''Materials and Procedure'''
+
:'''Materials and Procedure'''
-
 
+
-
 
+
-
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Transformation">the protocol(Transformation)</a></html>
+
 +
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Transformation">protocol9</a></html>
===Experiment:Electrophoresis===
===Experiment:Electrophoresis===
-
'''Member'''<br />
+
:'''Member'''<br />
-
naoto and watachin
+
:naoto and watachin
-
'''Materials and Procedure'''
+
:'''Materials and Procedure'''
-
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">the protocol(Electrophoresis)</a></html>
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">protocol4</a></html>
-
'''Result'''
+
:'''Result'''
[[Image:2010-09-23-04.jpg]]
[[Image:2010-09-23-04.jpg]]
-
===Experiment:DNA purification from agarose gel with QIAGEN===
+
===Experiment:DNA Extraction from agarose gel===
-
'''Member'''<br />
+
:'''Member'''<br />
-
naoto and watachin
+
:naoto and watachin
-
 
+
-
'''Materials and Procedure'''
+
 +
:'''Materials and Procedure'''
-
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">the protocol(DNA purification from agarose gel with QIAGEN)</a></html>
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">protocol5</a></html>

Latest revision as of 14:27, 27 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
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22 23 24 25 26 27 28
29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
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12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
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3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

2010/9/23 Thursday (watachin)

Experiment:PCR

Member
naoto
Materials and Procedure
E.coli introduced bcsA,B,C,D
follow protocol3

Experiment:Plating

Member
watachin
Materials and Procedure
plated E.coli(JM109) that transformated bcs genes(A.xlinum) to LB + cam medium from LB broth

Experiment:Colony PCR

Member
naoto
Materials and Procedure
Colony PCR production of bcs genes
follow protocol3

Experiment:Insert check Electrophoresis

Member
watachin
Materials and Procedure
follow protocol5
Result

2010-09-23-01.jpg 2010-09-23-02.jpg

Experiment:Electrophoresis of bcs genes

Member
naoto and watachin
Materials and Procedure
follow the protocol(Electrophoresis)

Experiment:DNA Extraction from agarose gel

Member
naoto and watachin
Materials and Procedure
follow protocol5

Experiment:Electrophoresis

Member
naoto and watachin
Materials and Procedure
  • Colony PCR production
follow protocol4
Result

2010-09-23-03.jpg

Experiment:Transformation

Member
watachin
Materials and Procedure
follow protocol9

Experiment:Electrophoresis

Member
naoto and watachin
Materials and Procedure
follow protocol4
Result

2010-09-23-04.jpg

Experiment:DNA Extraction from agarose gel

Member
naoto and watachin
Materials and Procedure
follow protocol5
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