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Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/17
From 2010.igem.org
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=2010/10/17= | =2010/10/17= | ||
===1:PCR=== | ===1:PCR=== | ||
| + | <Member><br /> | ||
| + | nito | ||
| + | |||
| + | <Sample>(temperature in annealing:℃)<br /> | ||
| + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2-7]<br /> | ||
| + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8-10] | ||
| + | |||
| + | <Protocol><br /> | ||
| + | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ] | ||
| + | |||
===2:DNA Ligation=== | ===2:DNA Ligation=== | ||
| + | ＜Member＞<br /> | ||
| + | Takahiro | ||
| + | |||
| + | ＜Sample＞<br /> | ||
| + | *bcsB-pvC | ||
| + | *bcsC-pvC | ||
| + | *bcsB-vector | ||
| + | *bcsC-vector | ||
| + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2-7]-vector | ||
| + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2-7]-vector | ||
| + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2]-[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 3] | ||
| + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4]-[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5] | ||
| + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2]-[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 3] | ||
| + | (after digestion) | ||
| + | |||
| + | ＜Protocol＞<br /> | ||
| + | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 3 ] | ||
| + | |||
===3:Electrophoresis=== | ===3:Electrophoresis=== | ||
<Protocol><br /> | <Protocol><br /> | ||
| Line 30: | Line 58: | ||
<Result><br /> | <Result><br /> | ||
| - | [[Image:]] | + | [[Image:Matsuura_2010-10-17_20hr_08min.JPG]] |
===6:PCR=== | ===6:PCR=== | ||
| Line 36: | Line 64: | ||
===8:DNA Digestion=== | ===8:DNA Digestion=== | ||
===9:Electrophoresis=== | ===9:Electrophoresis=== | ||
| + | <Protocol><br /> | ||
| + | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]<br /> | ||
| + | |||
| + | <Result><br /> | ||
| + | [[Image:Matsuura_2010-10-17_22hr_08min.JPG ]] | ||
| + | |||
===10:Electrophoresis=== | ===10:Electrophoresis=== | ||
| + | <Protocol><br /> | ||
| + | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]<br /> | ||
| + | |||
| + | <Result><br /> | ||
| + | [[Image:Matsuura_2010-10-18_01hr_41min.JPG]] | ||
| + | |||
===11:DNA extraction=== | ===11:DNA extraction=== | ||
===12:DNA extraction from gels=== | ===12:DNA extraction from gels=== | ||
| + | <Protocol><br /> | ||
| + | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]<br /> | ||
| + | |||
| + | <Result><br /> | ||
| + | [[Image:Matsuura_2010-10-18_03hr_10min.JPG]] | ||
| + | |||
===13:DNA extraction=== | ===13:DNA extraction=== | ||
===14:PCR=== | ===14:PCR=== | ||
Latest revision as of 14:07, 27 October 2010
E.coli Pattern Formation Project Notebook
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Contents |
2010/10/17
1:PCR
<Member>
nito
<Sample>(temperature in annealing:℃)
<Protocol>
See Protocol 1
2:DNA Ligation
＜Member＞
Takahiro
＜Sample＞
(after digestion)
＜Protocol＞
See Protocol 3
3:Electrophoresis
<Protocol>
See Protocol 8
<Result>
4:Electrophoresis
<Protocol>
See Protocol 8
<Result>
5:DNA extraction from gels
<Protocol>
See Protocol 4
<Result>
6:PCR
7:Transformation
8:DNA Digestion
9:Electrophoresis
<Protocol>
See Protocol 8
<Result>
10:Electrophoresis
<Protocol>
See Protocol 8
<Result>
11:DNA extraction
12:DNA extraction from gels
<Protocol>
See Protocol 4
<Result>
