Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/18

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==2010/09/18 Saturday (Naoto)==
==2010/09/18 Saturday (Naoto)==
===Experiment:Electrophoresis===
===Experiment:Electrophoresis===
-
'''member'''
+
:'''Member'''
-
naoto and easily
+
:naoto and easily
-
'''material'''
+
:'''Material'''
-
*PCR production(pSB1C3)
+
:*PCR production(pSB1C3)
-
If you want to know other materials,refer to protocol4  
+
:If you want to know other materials,refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
-
'''procedure'''
+
:'''Procedure'''
-
follow to protocol4
+
:follow to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol3</a></html>
-
'''result'''
+
:'''Result'''
-
Bands didn't appear
+
:Bands didn't appear
-
===Experiment:Insert check PCR===
+
===Experiment:Colony PCR===
-
'''member'''
+
:'''Member'''
-
naoto and easily
+
:naoto and easily
-
'''material'''
+
:'''Material'''
-
*Parts of bcsA,B,C,D(''A.xylinum''),bcsA,B,C(E.coli) in colonies of ''E,coli''
+
:*Parts of bcsA,B,C,D(''A.xylinum'') bcsA,B,C(''E.coli'')in colonies of ''E,coli''
-
If you want to know other materials,refer to protocol3
+
:If you want to know other materials,refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol3</a></html>
-
'''procedure'''
+
:'''Procedure'''
-
follow to protocol3
+
:follow to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol3</a></html>
-
===Experiment:Direct PCR===
+
===Experiment:PCR===
-
'''member'''
+
:'''Member'''
-
naoto and easily
+
:naoto and easily
-
'''material'''
+
:'''Material'''
-
*bcsA,B,C,D(''A.xylinum''),bcsA,B,C(E.coli),pSB1C3
+
:*bcsA,B,C,D(''A.xylinum''),pSB1C3
-
If you want to know other materials,refer to protocol3
+
:If you want to know other materials,refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol3</a></html>
-
'''procedure'''
+
:'''Procedure'''
-
follow to protocol3
+
:follow to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol3</a></html>
-
===Experiment:Electrophoresis of bcsA,B,C,D(''A.xylinum''),bcsA,B,C(E.coli),pSB1C3===
+
===Experiment:Electrophoresis of bcsA,B,C,D(''A.xylinum''),pSB1C3===
-
'''member'''
+
:'''Member'''
-
naoto and easily
+
:naoto and easily
-
'''material'''
+
:'''Material'''
-
*PCR production(bcsA,B,C,D(''A.xylinum''),bcsA,B,C(E.coli),pSB1C3)
+
:*PCR production(bcsA,B,C,D(''A.xylinum''),bcsA,B,C(E.coli),pSB1C3)
-
If you want to know other materials,refer to protocol4  
+
:If you want to know other materials,refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
-
'''procedure'''
+
:'''Procedure'''
-
follow to protocol4
+
:follow to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol3</a></html>
-
'''result'''
+
:'''Result'''
-
Only band of bcsA(''E.coli'') could be seen
+
:Only band of bcsA(''E.coli'') could be seen
-
===Experiment:Purification bcsA(''E.coli'')===
+
===Experiment:DNA Extraction from agarose gel===
-
'''member'''
+
:'''Member'''
-
naoto and easily
+
:naoto and easily
-
'''material'''
+
:'''Material'''
-
*bcsA(''E.coli'') in agarose gel
+
:*bcsA(''E.coli'') in agarose gel
-
If you want to know other materials,refer to protocol5
+
:If you want to know other materials,refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol5</a></html>
-
'''procedure'''
+
:'''Procedure'''
-
follow to protocol5
+
:follow to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol5</a></html>
-
===Experiment:Electrophoresis of insert check PCR===
+
===Experiment:Electrophoresis of Colony PCR===
-
'''member'''
+
:'''Member'''
-
naoto and easily
+
:naoto and easily
-
'''material'''
+
:'''Material'''
-
*PCR production (parts of bcsA,B,C,D(''A.xylinum''),bcsA,B,C(E.coli) in colonies of ''E,coli'')
+
:*PCR production (parts of bcsA,B,C,D(''A.xylinum'') and bcsA(''E.coli'')in colonies of ''E,coli'')
-
If you want to know other materials,refer to protocol4
+
:If you want to know other materials,refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
-
'''procedure'''
+
:'''Procedure'''
 +
:refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
-
follow to protocol4
+
:'''Result'''
-
'''result'''
+
:Bands of bcsB,C(''A.xylinum'') appreared
 +
:*1~8  bcsA(''A.xylinum'')
 +
:*9~12  bcsB(''A.xylinum'')
 +
:*13    bcsC(''A.xylinum'')
 +
:*14~15 bcsD(''A.xylinum'')
 +
:*16~20 bcsA(''E.coli'')
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Bands of bcsB,C(''A.xylinum'') appreared
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<br/>
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*1~8  bcsA(''A.xylinum'')
+
-
*9~12  bcsB(''A.xylinum'')
+
-
*13    bcsC(''A.xylinum'')
+
-
*14~15 bcsD(''A.xylinum'')
+
-
*16~20 bcsA(''E.coli'')
+

Latest revision as of 07:11, 27 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
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September 2010
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October 2010
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31

2010/09/18 Saturday (Naoto)

Experiment:Electrophoresis

Member
naoto and easily
Material
  • PCR production(pSB1C3)
If you want to know other materials,refer to protocol4
Procedure
follow to protocol3
Result
Bands didn't appear

Experiment:Colony PCR

Member
naoto and easily
Material
  • Parts of bcsA,B,C,D(A.xylinum) bcsA,B,C(E.coli)in colonies of E,coli
If you want to know other materials,refer to protocol3
Procedure
follow to protocol3

Experiment:PCR

Member
naoto and easily
Material
  • bcsA,B,C,D(A.xylinum),pSB1C3
If you want to know other materials,refer to protocol3
Procedure
follow to protocol3

Experiment:Electrophoresis of bcsA,B,C,D(A.xylinum),pSB1C3

Member
naoto and easily
Material
  • PCR production(bcsA,B,C,D(A.xylinum),bcsA,B,C(E.coli),pSB1C3)
If you want to know other materials,refer to protocol4
Procedure
follow to protocol3
Result
Only band of bcsA(E.coli) could be seen

Experiment:DNA Extraction from agarose gel

Member
naoto and easily
Material
  • bcsA(E.coli) in agarose gel
If you want to know other materials,refer to protocol5
Procedure
follow to protocol5

Experiment:Electrophoresis of Colony PCR

Member
naoto and easily
Material
  • PCR production (parts of bcsA,B,C,D(A.xylinum) and bcsA(E.coli)in colonies of E,coli)
If you want to know other materials,refer to protocol4
Procedure
refer to protocol4
Result
Bands of bcsB,C(A.xylinum) appreared
  • 1~8 bcsA(A.xylinum)
  • 9~12 bcsB(A.xylinum)
  • 13 bcsC(A.xylinum)
  • 14~15 bcsD(A.xylinum)
  • 16~20 bcsA(E.coli)

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Fiber/2010/09/18"