Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/13

From 2010.igem.org

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{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
__NOTOC__
__NOTOC__
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==2010/9/13 Monday (Naoto)==
+
==2010/09/13 Monday (Naoto)==
===Experiment:direct PCR===
===Experiment:direct PCR===
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'''member'''
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'''Member'''
naoto
naoto
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'''material'''
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'''Material'''
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*bcsA,B,C(''A.xylinum'')and bcsB(''E.coli'') 0.5µl
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*bcsA,B,C
If you want to know other materials, see protocol3
If you want to know other materials, see protocol3
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'''procedure'''
+
'''Procedure'''
follow protocol3
follow protocol3
===Experiment:electrophoresis===
===Experiment:electrophoresis===
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'''member'''
+
:'''Member'''
-
naoto
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:naoto
-
'''material'''
+
:'''Material'''
-
*PCR production(see above)
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:*PCR production(see above)
-
If you want to know other materials, see protocol4
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:If you want to know other materials, see protocol4
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'''procedure'''
+
:'''Procedure'''
-
follow protocol4
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:follow protocol4
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'''result'''
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:'''result'''
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bands of bcsA,C(''A.xylinum'')and bcsB(''E.coli'')is appear clearly
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:bands of bcsA,C(''A.xylinum'')and appeared clearly
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===Experiment:Purification from agarose gel with QIAGEN===
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===Experiment:DNA Extraction from agarose gel===
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'''member'''
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:'''Member'''
-
naoto
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:naoto
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'''material'''
+
:'''Material'''
-
*bcsA,C(''A.xylinum'')and bcsB(''E.coli'') in agarose gel
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:*bcsA,C(''A.xylinum'')in agarose gel
-
If you want to know other materials, see protocol5
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:If you want to know other materials, see protocol5
-
'''procedure'''
+
:'''Procedure'''
-
follow protocol5
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:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5</a></html>

Revision as of 06:48, 27 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
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September 2010
SUNMONTUEWEDTHUFRISAT
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October 2010
SUNMONTUEWEDTHUFRISAT
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31

2010/09/13 Monday (Naoto)

Experiment:direct PCR

Member

naoto

Material

  • bcsA,B,C

If you want to know other materials, see protocol3

Procedure

follow protocol3

Experiment:electrophoresis

Member
naoto
Material
  • PCR production(see above)
If you want to know other materials, see protocol4
Procedure
follow protocol4
result
bands of bcsA,C(A.xylinum)and appeared clearly

Experiment:DNA Extraction from agarose gel

Member
naoto
Material
  • bcsA,C(A.xylinum)in agarose gel
If you want to know other materials, see protocol5
Procedure
follow protocol5
Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Fiber/2010/09/13"