Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/09

From 2010.igem.org

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==2010/09/09 Thursday (Naoto)==
==2010/09/09 Thursday (Naoto)==
===Experiment:Ligation===
===Experiment:Ligation===
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'''member'''
+
:'''Member'''
-
naoto and NEX
+
:naoto and NEX
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'''material'''
+
:'''Material'''
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*bcsA,B(from ''A.xylinum'') 10µl each
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:*bcsA,B 10µl each
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*bcsC,D(from ''A.xylinum'') 10µl each
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:*bcsC,D 10µl each
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*bcsA,B(from ''E.coli'') 10µl each
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:*bcsA,B 10µl each
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*Ligation Covinience Kit 20µl each
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:*ligation Covinience Kit 20µl each
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'''procedure'''
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:'''Procedure'''
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#add material to PCR tube
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:#Add material to PCR tube
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#incubate (20℃　10min)
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:#Incubate (20℃　10min)
===Experiment:Electrophoresis===
===Experiment:Electrophoresis===
-
'''member'''
+
:'''Member'''
-
naoto and NEX
+
:naoto and NEX
-
'''material'''
+
:'''Material'''
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*ligation products(see above)
+
:*ligation products(see above)
-
we show other material at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
+
:we show other material at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
-
'''procedure'''
+
:'''Procedure'''
-
see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
+
:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
 +
 
 +
<br/>

Latest revision as of 06:41, 27 October 2010


E.coli Fiber Project Notebook

August 2010
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September 2010
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October 2010
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2010/09/09 Thursday (Naoto)

Experiment:Ligation

Member
naoto and NEX
Material
  • bcsA,B 10µl each
  • bcsC,D 10µl each
  • bcsA,B 10µl each
  • ligation Covinience Kit 20µl each
Procedure
  1. Add material to PCR tube
  2. Incubate (20℃　10min)

Experiment:Electrophoresis

Member
naoto and NEX
Material
  • ligation products(see above)
we show other material at protocol4
Procedure
see protocol4

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Fiber/2010/09/09"