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Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/24
From 2010.igem.org
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===Experiment:Agarose Gels Electrophoresis=== | ===Experiment:Agarose Gels Electrophoresis=== | ||
| - | '''member''' | + | :'''member''' |
| - | NEX , bambi75 and watachin | + | :NEX , bambi75 and watachin |
| - | '''Materials,Procedure''' | + | :'''Materials,Procedure''' |
| - | Same protocol on August 16th. | + | :Same protocol on August 16th. |
| - | PCR Product of insert are bcsA and bcsC. | + | :PCR Product of insert are bcsA and bcsC. |
===Experiment:PCR=== | ===Experiment:PCR=== | ||
| - | ''' | + | :'''Member''' |
| - | NEX , bambi75 and watachin | + | :NEX , bambi75 and watachin |
| - | ''' | + | :'''Material''' |
| - | *sterilized water 213μl | + | :*sterilized water 213μl |
| - | *Ex taq buffer 30μl | + | :*Ex taq buffer 30μl |
| - | *dNTP 24μl | + | :*dNTP 24μl |
| - | *Ex taq 3μl | + | :*Ex taq 3μl |
| - | * | + | :*bcsA sense 15μl |
| - | * | + | :*bcsA antisense 15μl |
| - | '''Procedure''' | + | :'''Procedure''' |
| - | + | ||
| - | + | ||
| + | :Please see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html> | ||
===Experiment:Restriction enzyme digestion=== | ===Experiment:Restriction enzyme digestion=== | ||
| - | ''' | + | :'''Member''' |
| - | Same above | + | :Same above |
| - | '''Materials''' | + | :'''Materials''' |
| - | *PCR production | + | :*PCR production |
| - | * | + | :*M buffer 30μl |
| - | *BSA 30μl | + | :*BSA 30μl |
| - | *Xba1 6μl | + | :*Xba1 6μl |
| - | *Spe1 6μl | + | :*Spe1 6μl |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| + | :'''Procedure''' | ||
| + | :Please see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80 restriction enzyme digestion">protocol7</a></html> | ||
===Experiment:Transformation=== | ===Experiment:Transformation=== | ||
| - | ''' | + | :'''Member''' |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| + | :Same above | ||
| + | :'''Materials , Procedure''' | ||
| + | :Please refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80 trasnformation">protocol9</a></html> | ||
<br/> | <br/> | ||
Latest revision as of 21:45, 26 October 2010
E.coli Fiber Project Notebook
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2010/08/24 Tuesday(bambi75)
Experiment:Agarose Gels Electrophoresis
- member
- NEX , bambi75 and watachin
- Materials,Procedure
- Same protocol on August 16th.
- PCR Product of insert are bcsA and bcsC.
Experiment:PCR
- Member
- NEX , bambi75 and watachin
- Material
- sterilized water 213μl
- Ex taq buffer 30μl
- dNTP 24μl
- Ex taq 3μl
- bcsA sense 15μl
- bcsA antisense 15μl
- Procedure
- Please see protocol3
Experiment:Restriction enzyme digestion
- Member
- Same above
- Materials
- PCR production
- M buffer 30μl
- BSA 30μl
- Xba1 6μl
- Spe1 6μl
- Procedure
- Please see protocol7
Experiment:Transformation
- Member
- Same above
- Materials , Procedure
- Please refer to protocol9
