Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/13

(Difference between revisions)

Latest revision as of 21:28, 26 October 2010

E.coli Fiber Project Notebook

Member

NEX , bambi75 and watachin

Materials

Procedure

mix materials : M buffer(5μl) , BSA1(5μl) , Xba1(1μl) and Spe1 (1μl) to PCR products(50μl). Repeat this process 3 times , for 3 PCR products.
incubation(37℃).

@@ Line 1: / Line 1: @@
-{{:Team:Tokyo_Metropolitan/Header}}
+{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
-===Experiment:restriction enzyme digestion===
+==2010/08/13 Friday (bambi75)==
-====member====
+===Experiment:Restrict enzyme digestion===
-NEX , bambi75 and watachin
-===Materials===
-*PCR products  50μl×3
-*m buffer  15μl
-*BSA 1 15μl
-*Xba1  3μl
-*SPE1  3μl
-===Equipment===
+:'''Member'''
-*pipet
-*pipet tip
-===Procedure===
+:NEX , bambi75 and watachin
-)mix materials : m buffer(5μl) , BSA1(5μl) , Xba1(1μl) and SPE1 (1μl) to PCR products(50μl). Repeat this process 3 times , for 3 PCR products.
-)incubation(37℃).
+:'''Materials'''
+:*bcsA,B,C,D(each):50μl
+:*10×M buffer:15μl
+:*BSA:15μl
+:*XbaI:3μl
+:*SpeI:3μl
+:'''Procedure'''
+:#mix materials : M buffer(5μl) , BSA1(5μl) , Xba1(1μl) and Spe1 (1μl) to PCR products(50μl). Repeat this process 3 times , for 3 PCR products.
+:#incubation(37℃).
+<br />