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Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/13
From 2010.igem.org
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==2010/08/13 Friday (bambi75)== | ==2010/08/13 Friday (bambi75)== | ||
| - | ===Experiment: | + | ===Experiment:Restrict enzyme digestion=== |
| - | ''' | + | :'''Member''' |
| - | NEX , bambi75 and watachin | + | :NEX , bambi75 and watachin |
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| + | :'''Materials''' | ||
| + | :*bcsA,B,C,D(each):50μl | ||
| + | :*10×M buffer:15μl | ||
| + | :*BSA:15μl | ||
| + | :*XbaI:3μl | ||
| + | :*SpeI:3μl | ||
| + | :'''Procedure''' | ||
| + | :#mix materials : M buffer(5μl) , BSA1(5μl) , Xba1(1μl) and Spe1 (1μl) to PCR products(50μl). Repeat this process 3 times , for 3 PCR products. | ||
| + | :#incubation(37℃). | ||
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Latest revision as of 21:28, 26 October 2010
E.coli Fiber Project Notebook
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2010/08/13 Friday (bambi75)
Experiment:Restrict enzyme digestion
- Member
- NEX , bambi75 and watachin
- Materials
- bcsA,B,C,D(each):50μl
- 10×M buffer:15μl
- BSA:15μl
- XbaI:3μl
- SpeI:3μl
- Procedure
- mix materials : M buffer(5μl) , BSA1(5μl) , Xba1(1μl) and Spe1 (1μl) to PCR products(50μl). Repeat this process 3 times , for 3 PCR products.
- incubation(37℃).
