Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/13

From 2010.igem.org

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(Experiment:Restrict enzyme digestion)
 
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==2010/08/13 Friday (bambi75)==
==2010/08/13 Friday (bambi75)==
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===Experiment:Digestion===
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===Experiment:Restrict enzyme digestion===
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'''member'''
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:'''Member'''
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NEX , bambi75 and watachin
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:NEX , bambi75 and watachin
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:'''Materials'''
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:*bcsA,B,C,D(each):50μl
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:*10×M buffer:15μl
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:*BSA:15μl
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:*XbaI:3μl
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:*SpeI:3μl
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'''Materials'''
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:'''Procedure'''
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*PCR products 50μl×3
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:#mix materials : M buffer(5μl) , BSA1(5μl) , Xba1(1μl) and Spe1 (1μl) to PCR products(50μl). Repeat this process 3 times , for 3 PCR products.
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*m buffer  15μl
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:#incubation(37℃).
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*BSA 15μl
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*Xba1  3μl
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*Spe1  3μl
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<br />
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'''Procedure'''
+
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#mix materials : m buffer(5μl) , BSA1(5μl) , Xba1(1μl) and SPE1 (1μl) to PCR products(50μl). Repeat this process 3 times , for 3 PCR products.
+
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#incubation(37℃).
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Latest revision as of 21:28, 26 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
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29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
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26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
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31

2010/08/13 Friday (bambi75)

Experiment:Restrict enzyme digestion

Member
NEX , bambi75 and watachin
Materials
  • bcsA,B,C,D(each):50μl
  • 10×M buffer:15μl
  • BSA:15μl
  • XbaI:3μl
  • SpeI:3μl
Procedure
  1. mix materials : M buffer(5μl) , BSA1(5μl) , Xba1(1μl) and Spe1 (1μl) to PCR products(50μl). Repeat this process 3 times , for 3 PCR products.
  2. incubation(37℃).