Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/13

From 2010.igem.org

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(Experiment:Restrict enzyme digestion)
 
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{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
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===Experiment:restriction enzyme digestion===
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==2010/08/13 Friday (bambi75)==
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====member====
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===Experiment:Restrict enzyme digestion===
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NEX , bambi75 and watachin
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===Materials===
 
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*PCR products  50μl×3
 
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*m buffer  15μl
 
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*BSA 1 15μl
 
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*Xba1  3μl
 
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*SPE1  3μl
 
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===Equipment===
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:'''Member'''
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*pipet
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*pipet tip
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===Procedure===
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:NEX , bambi75 and watachin
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1)mix materials : m buffer(5μl) , BSA1(5μl) , Xba1(1μl) and SPE1 (1μl) to PCR products(50μl). Repeat this process 3 times , for 3 PCR products.
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2)incubation(37℃).
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:'''Materials'''
 +
:*bcsA,B,C,D(each):50μl
 +
:*10×M buffer:15μl
 +
:*BSA:15μl
 +
:*XbaI:3μl
 +
:*SpeI:3μl
 +
 
 +
:'''Procedure'''
 +
:#mix materials : M buffer(5μl) , BSA1(5μl) , Xba1(1μl) and Spe1 (1μl) to PCR products(50μl). Repeat this process 3 times , for 3 PCR products.
 +
:#incubation(37℃).
 +
 
 +
<br />

Latest revision as of 21:28, 26 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
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September 2010
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October 2010
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2010/08/13 Friday (bambi75)

Experiment:Restrict enzyme digestion

Member
NEX , bambi75 and watachin
Materials
  • bcsA,B,C,D(each):50μl
  • 10×M buffer:15μl
  • BSA:15μl
  • XbaI:3μl
  • SpeI:3μl
Procedure
  1. mix materials : M buffer(5μl) , BSA1(5μl) , Xba1(1μl) and Spe1 (1μl) to PCR products(50μl). Repeat this process 3 times , for 3 PCR products.
  2. incubation(37℃).