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Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/20
From 2010.igem.org
(Difference between revisions)
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'''member''' | '''member''' | ||
naoto and watachin | naoto and watachin | ||
| + | |||
| + | ===Experiment:Colony PCR=== | ||
| + | '''material''' | ||
| + | |||
| + | *bcsB | ||
| + | *bcsC | ||
| + | other materials were same as <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html> | ||
| + | |||
| + | '''procedure''' | ||
| + | |||
| + | see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html> | ||
===Experiment:Sequence=== | ===Experiment:Sequence=== | ||
'''material''' | '''material''' | ||
| - | + | *bcsD in pSB1C3(No.89~96) | |
| - | + | *Big Dye | |
| - | + | *primer | |
| - | + | *DW | |
| - | + | *ethanol | |
| - | + | *EDTA | |
| - | + | ||
| - | + | ||
'''procedure''' | '''procedure''' | ||
Revision as of 06:58, 26 October 2010
E.coli Fiber Project Notebook
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2010/10/20 Wednesday (Naoto)
member naoto and watachin
Experiment:Colony PCR
material
- bcsB
- bcsC
other materials were same as protocol3
procedure
see protocol3
Experiment:Sequence
material
- bcsD in pSB1C3(No.89~96)
- Big Dye
- primer
- DW
- ethanol
- EDTA
procedure
- mix materials(DNA<50ng)
- PCR
- Add EDTA and Ethanol and put at room temperature (15min)
- centrifuge (8000rpm,30min)
- throw away supernatant
- add ethanol again
- centrifuge (8000rpm,15min)
- put at refrigerator
- throw away supernatant
- add Hi-Di solution
- transfer these sample to plate for sequence
- read sequence
result
Failure to read sequence
