"
Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/23
From 2010.igem.org
(Difference between revisions)
| Line 18: | Line 18: | ||
see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html> | see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html> | ||
| - | |||
'''result''' | '''result''' | ||
| Line 29: | Line 28: | ||
seem to be correct inserts | seem to be correct inserts | ||
| + | |||
| + | ===Preparation:making preculture=== | ||
| + | '''material''' | ||
| + | *Colony of No.54,64,75 | ||
| + | *LB+Cam Broth | ||
| + | |||
| + | '''procedure''' | ||
| + | |||
| + | *Pick up a colony and inoculate into LB+Cam broth | ||
Revision as of 16:22, 25 October 2010
E.coli Fiber Project Notebook
|
|
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2010/10/23 Saturday (Naoto)
member
naoto and watachin
Experiment:Colony PCR
material
- colony of E.coli
- bcsB:1~32
- bcsC:33~64
- bcsD:65~96
other materials were same as protocol3
procedure
see protocol3
result
From the length of bands
bcsC→No.54 and 64
bcsD→No.75
seem to be correct inserts
Preparation:making preculture
material
- Colony of No.54,64,75
- LB+Cam Broth
procedure
- Pick up a colony and inoculate into LB+Cam broth
