Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/06

From 2010.igem.org

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===1:PCR===
===1:PCR===
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<Sample><br />
<Sample><br />
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:*1(BBa_K208017)
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:*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1]
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:*2(BBa_JM109)
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:*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2]
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:*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5]
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<Samlpe><br />
<Samlpe><br />
PCR products.
PCR products.
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*1
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1]
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*2
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2]
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5]
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7]
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8]
<Protocol><br />
<Protocol><br />
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<Sample><br />
<Sample><br />
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7]
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8]
<Protocol>
<Protocol>
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 3 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 3 ]

Latest revision as of 18:14, 24 October 2010


E.coli Pattern Formation Project Notebook



August 2010
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September 2010
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Contents

2010/09/06

1:PCR

<Member>
Hitomi,nito


<Sample>


<Protocol>
See Protocol 2

Tubes(Temperature in annealing)

  • 1(BBa_K208017) 71.5,72.0,72.5℃
  • 2(BBa_JM109) 71.0,71.5,72.0℃
  • 5(BBa_K208017) 70.0,70.5,71.0℃

2:DNA extraction

<Member>
Hitomi


<Protocol>
See Protocol 4


3:Electrophoresis

<Samlpe>
PCR products.

<Protocol>
See Protocol 8


<Result>

Matsuura 2010-08-06 20hr 02min (16).JPG

4:Ligation

<Membber>
nito


<Sample>


<Protocol> See Protocol 3