Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/06

From 2010.igem.org

(Difference between revisions)
(→4:Ligation)
 
(17 intermediate revisions not shown)
Line 1: Line 1:
 +
{{:Team:Tokyo_Metropolitan/Notebook/Pattern}}
 +
=2010/09/06=
=2010/09/06=
-
===1:PCR===
 
-
<Member>
 
 +
===1:PCR===
 +
<Member><br />
Hitomi,nito
Hitomi,nito
-
<Sample>
+
<Sample><br />
-
:*1(BBa_K208017)
+
:*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1]
-
:*2(BBa_JM109)
+
:*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2]
-
:*5(BBa_K208017)
+
:*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5]
-
<Protocol>
+
<Protocol><br />
-
 
+
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ]
-
See Protocol2
+
-
 
+
-
(https://2010.igem.org/Team:Tokyo_Metroporitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/)
+
-
 
+
Tubes(Temperature in annealing)
Tubes(Temperature in annealing)
Line 24: Line 22:
:*5(BBa_K208017) 70.0,70.5,71.0℃
:*5(BBa_K208017) 70.0,70.5,71.0℃
-
===2:Purification of PCR===
+
===2:DNA extraction===
-
 
+
<Member><br />
-
<Member>
+
-
 
+
Hitomi
Hitomi
-
<Protocol>
+
<Protocol><br />
 +
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
-
See Protocol
 
===3:Electrophoresis===
===3:Electrophoresis===
-
<Samlpe>
+
<Samlpe><br />
-
 
+
PCR products.
PCR products.
-
:*1
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1]
-
:*2
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2]
-
:*5
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5]
-
:*7
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7]
-
:*8
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8]
 +
<Protocol><br />
 +
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
-
<Protocol>
 
-
See Protocol8
+
<Result>
-
(https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/)
+
[[image:Matsuura_2010-08-06_20hr_02min_(16).JPG]]
-
 
+
-
 
+
-
<Result>
+
===4:Ligation===
===4:Ligation===
-
 
+
<Membber><br />
-
 
+
-
<Membber>
+
-
 
+
nito
nito
-
<Sample>
+
<Sample><br />
-
 
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7]
-
:*7
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8]
-
:*8
+
<Protocol>
<Protocol>
-
 
+
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 3 ]
-
See Protocol10
+

Latest revision as of 18:14, 24 October 2010


E.coli Pattern Formation Project Notebook



August 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

Contents

2010/09/06

1:PCR

<Member>
Hitomi,nito


<Sample>


<Protocol>
See Protocol 2

Tubes(Temperature in annealing)

  • 1(BBa_K208017) 71.5,72.0,72.5℃
  • 2(BBa_JM109) 71.0,71.5,72.0℃
  • 5(BBa_K208017) 70.0,70.5,71.0℃

2:DNA extraction

<Member>
Hitomi


<Protocol>
See Protocol 4


3:Electrophoresis

<Samlpe>
PCR products.

<Protocol>
See Protocol 8


<Result>

Matsuura 2010-08-06 20hr 02min (16).JPG

4:Ligation

<Membber>
nito


<Sample>


<Protocol> See Protocol 3

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Pattern/2010/09/06"