Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/06

From 2010.igem.org

(Difference between revisions)
(New page: =2010/09/06= ===1:PCR=== <Member> Hitomi,nito <Sample> :*1(BBa_K208017) :*2(BBa_JM109) :*5(BBa_K208017) <Protocol> See Protocol2 (https://2010.igem.org/Team:Tokyo_Metroporitan/Projec...)
 
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{{:Team:Tokyo_Metropolitan/Notebook/Pattern}}
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=2010/09/06=
=2010/09/06=
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===1:PCR===
 
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<Member>
 
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===1:PCR===
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<Member><br />
Hitomi,nito
Hitomi,nito
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<Sample>
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<Sample><br />
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:*1(BBa_K208017)
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:*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1]
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:*2(BBa_JM109)
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:*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2]
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:*5(BBa_K208017)
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:*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5]
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<Protocol>
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<Protocol><br />
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See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ]
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See Protocol2
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(https://2010.igem.org/Team:Tokyo_Metroporitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/)
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Tubes(Temperature in annealing)
Tubes(Temperature in annealing)
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:*5(BBa_K208017) 70.0,70.5,71.0℃
:*5(BBa_K208017) 70.0,70.5,71.0℃
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===2:Purification of PCR===
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===2:DNA extraction===
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<Member><br />
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<Member>
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-
 
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Hitomi
Hitomi
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<Protocol>
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<Protocol><br />
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See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
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See Protocol
 
===3:Electrophoresis===
===3:Electrophoresis===
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<Samlpe>
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<Samlpe><br />
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PCR products.
PCR products.
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:*1
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1]
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:*2
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2]
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:*5
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5]
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:*7
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7]
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:*8
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8]
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<Protocol><br />
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See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
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<Protocol>
 
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See Protocol8
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<Result>
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(https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/)
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[[image:Matsuura_2010-08-06_20hr_02min_(16).JPG]]
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<Result>
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===4:Ligation===
===4:Ligation===
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<Membber><br />
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<Membber>
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-
 
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nito
nito
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<Sample>
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<Sample><br />
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7]
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:*7
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8]
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:*8
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<Protocol>
<Protocol>
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See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 3 ]
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See Protocol
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Latest revision as of 18:14, 24 October 2010


E.coli Pattern Formation Project Notebook



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September 2010
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Contents

2010/09/06

1:PCR

<Member>
Hitomi,nito


<Sample>


<Protocol>
See Protocol 2

Tubes(Temperature in annealing)

  • 1(BBa_K208017) 71.5,72.0,72.5℃
  • 2(BBa_JM109) 71.0,71.5,72.0℃
  • 5(BBa_K208017) 70.0,70.5,71.0℃

2:DNA extraction

<Member>
Hitomi


<Protocol>
See Protocol 4


3:Electrophoresis

<Samlpe>
PCR products.

<Protocol>
See Protocol 8


<Result>

Matsuura 2010-08-06 20hr 02min (16).JPG

4:Ligation

<Membber>
nito


<Sample>


<Protocol> See Protocol 3

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Pattern/2010/09/06"