Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/16

From 2010.igem.org

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{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
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==PCR/pBS1C3==
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==2010/09/16 Thuesday (Bambi75)==
 +
 
 +
===Experiment:PCR===
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'''member'''
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===member===
 
naoto and bambi75
naoto and bambi75
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===materials,Procedure===
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'''materials,Procedure'''
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Same on August 23th.
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 +
Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
*95℃3min
*95℃3min
*96℃1min
*96℃1min
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（96℃～72℃/30cycle）
（96℃～72℃/30cycle）
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==Agarose Gels Electro-phoresis==
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===Experiment:Agarose Gels Electro-phoresis===
 +
 
 +
'''member'''
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===member===
 
Same above
Same above
-
===Materials,Procedure===
+
 
 +
'''Materials,Procedure'''
Same protocol on August 16th.
Same protocol on August 16th.
PCR Product of insert is pBS1C3
PCR Product of insert is pBS1C3
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==Experiment:Make Agarose Gels Electro-phoresis==
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===Experiment:Experiment:Make Agarose Gels Electro-phoresis===
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===Member===
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'''Member'''
 +
 
Same above  
Same above  
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===Materials and Procedure===
 
-
Same protocol on August 16th.
 
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==Medium making==
 
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===member===
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'''Materials and Procedure'''
 +
 
 +
Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
 +
 
 +
===Preparation:Medium making===
 +
 
 +
'''member'''
 +
 
Same above
Same above
-
===materials and procedure===
+
'''materials and procedure'''
-
Same protocol on August 10th.
+
 
 +
Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol1</a></html>
 +
 
 +
==Experiment:Ligation==
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==Ligation==
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'''member'''
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===member===
 
Same above
Same above
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===materials and procedure===
+
'''materials and procedure'''
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follow the protocol(Ligation).
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follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol8</a></html>
*bcsA,B and C(E.coli).
*bcsA,B and C(E.coli).
*bcsA,B,C and D(A.xylium)
*bcsA,B,C and D(A.xylium)
*pSB1C3
*pSB1C3

Revision as of 17:14, 24 October 2010


E.coli Fiber Project Notebook

August 2010
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September 2010
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October 2010
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Contents

2010/09/16 Thuesday (Bambi75)

Experiment:PCR

member

naoto and bambi75

materials,Procedure

Same protocol4

  • 95℃3min
  • 96℃1min
  • 51℃1min
  • 72℃3min
  • 10℃∞

（96℃～72℃/30cycle）

Experiment:Agarose Gels Electro-phoresis

member

Same above

Materials,Procedure Same protocol on August 16th. PCR Product of insert is pBS1C3

Experiment:Experiment:Make Agarose Gels Electro-phoresis

Member

Same above

Materials and Procedure

Same protocol4

Preparation:Medium making

member

Same above

materials and procedure

Same protocol1


Experiment:Ligation

member

Same above

materials and procedure follow protocol8

  • bcsA,B and C(E.coli).
  • bcsA,B,C and D(A.xylium)
  • pSB1C3
Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Fiber/2010/09/16"