Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/23
From 2010.igem.org
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'''Procedure''' | '''Procedure''' | ||
- | + | see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">the protocol3</a></html> | |
===Experiment:Restriction enzyme digestion=== | ===Experiment:Restriction enzyme digestion=== |
Revision as of 16:54, 24 October 2010
![](https://static.igem.org/mediawiki/2010/9/9f/Re-main.png)
E.coli Fiber Project Notebook
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2010/8/23 Monday (watachin)
Experiment:Direct PCR(K12-bcsA,K12-bcsC)
Member
Bambi75 and watachin
Materials
- E.coli K12 colony
- 10µM/l forward Primer 5µl(K12-bcsA,K12-bcsC)
- 10µM/l reverse Primer 5µl(K12-bcsA,K12-bcsC)
- 10×EX taq buffer 10µl
- 2.5mM each dNTP mixture 10µl
- EX taq polymerase 1µl
- milli-Q 71µl
Procedure
see the protocol3
Experiment:Restriction enzyme digestion
Member
Bambi75 and watachin
material
- bcsA
- bcsC
You can see other material at the protocol6
procedure
see the protocol6