Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/13

From 2010.igem.org

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==2010/08/13 Friday (bambi75)==
==2010/08/13 Friday (bambi75)==
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===Experiment:Digestion of bcs===
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===Experiment:Digestion===

Revision as of 16:42, 24 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
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September 2010
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October 2010
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2010/08/13 Friday (bambi75)

Experiment:Digestion

member

NEX , bambi75 and watachin


Materials

  • PCR products 50μl×3
  • m buffer 15μl
  • BSA 15μl
  • Xba1 3μl
  • Spe1 3μl


Procedure

  1. mix materials : m buffer(5μl) , BSA1(5μl) , Xba1(1μl) and Spe1 (1μl) to PCR products(50μl). Repeat this process 3 times , for 3 PCR products.
  2. incubation(37℃).