Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/03

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Revision as of 08:24, 24 October 2010


E.coli Fiber Project Notebook

August 2010
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Contents

2010/10/03 Sunday (Naoto)

Experiment:PCR of bcsA(A.xylinum)

member

naoto and watachin

marerial

  • bcsA(A.xylinum)

If you want to know other materials, see protocol3

procedure

see protocol3


Experiment:Electrophoresis of bcsC,D(A.xylinum),bcsA,B,C,EFG,Q(E.coli),and pSB13

member

naoto and watachin

marerial

  • bcsC,D(A.xylinum),bcsA,B,C,EFG,Q(E.coli),pSB13

If you want to know other materials, see protocol4


procedure

see protocol4


Experiment:Miniprep of BBa_K145001

member

naoto and watachin

marerial

  • Preculture of E.coli having BBa_K145001

If you want to know other materials, see protocol9


procedure

see protocol9


Experiment:Ligation of pSB1C3 and bcsC,D(A.xylinum),bcsA(E.coli)

member

naoto and watachin

marerial

  • pSB1C3
  • bcsC,D(A.xylinum),bcsA(E.coli)

If you want to know other materials, see protocol7


procedure

see protocol4


Experiment:Transformation of parts of bcsC,D(A.xylinum),bcsA(E.coli)

member

naoto and watachin

marerial

  • parts of bcsC,D(A.xylinum),bcsA(E.coli)

If you want to know other materials, see protocol8


procedure

see protocol8


Experiment:Electrophoresis of bcsA(A.xylinum)

member

naoto and watachin

marerial

  • bcsA(A.xylinum)

If you want to know other materials, see protocol4


procedure

see protocol4


result

Band of bcsA could be seen clearly

Experiment:Purification of bcsA(A.xylinum)

member

naoto and watachin

marerial

  • bcsA(A.xylinum) in agarose gel

If you want to know other materials, see protocol5


procedure

see protocol4 protocol4