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Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/24
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Please refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80 trasnformation">protocol9</a></html> | Please refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80 trasnformation">protocol9</a></html> | ||
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Revision as of 20:37, 23 October 2010
E.coli Fiber Project Notebook
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Contents |
2010/08/24 Tuesday(bambi75)
Experiment:Agarose Gels Electrophoresis
member
NEX , bambi75 and watachin
Materials,Procedure
Same protocol on August 16th. PCR Product of insert are bcsA and bcsC.
Experiment:PCR
member
NEX , bambi75 and watachin
material
- sterilized water 213μl
- Ex taq buffer 30μl
- dNTP 24μl
- Ex taq 3μl
- K12bcsA sense 15μl
- K12bcsA antisense 15μl
Procedure
Please see protocol3
Experiment:Restriction enzyme digestion
member
Same above
Materials
- PCR production
- m buffer 30μl
- BSA 30μl
- Xba1 6μl
- Spe1 6μl
Procedure
Please see protocol4
Experiment:Transformation
member
Same above
Materials , Procedure
Please refer to protocol9
