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Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/24
From 2010.igem.org
(Difference between revisions)
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==2010/08/24 Tuesday(bambi75)== | ==2010/08/24 Tuesday(bambi75)== | ||
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===Experiment:Agarose Gels Electrophoresis=== | ===Experiment:Agarose Gels Electrophoresis=== | ||
'''member''' | '''member''' | ||
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NEX , bambi75 and watachin | NEX , bambi75 and watachin | ||
'''Materials,Procedure''' | '''Materials,Procedure''' | ||
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Same protocol on August 16th. | Same protocol on August 16th. | ||
PCR Product of insert are bcsA and bcsC. | PCR Product of insert are bcsA and bcsC. | ||
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'''Procedure''' | '''Procedure''' | ||
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Please see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html> | Please see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html> | ||
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| - | ==Experiment:Transformation== | + | ===Experiment:Transformation=== |
'''member''' | '''member''' | ||
Revision as of 18:41, 22 October 2010
E.coli Fiber Project Notebook
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Contents |
2010/08/24 Tuesday(bambi75)
Experiment:Agarose Gels Electrophoresis
member
NEX , bambi75 and watachin
Materials,Procedure
Same protocol on August 16th. PCR Product of insert are bcsA and bcsC.
Experiment:PCR
member
NEX , bambi75 and watachin
material
- sterilized water 213μl
- Ex taq buffer 30μl
- dNTP 24μl
- Ex taq 3μl
- K12bcsA sense 15μl
- K12bcsA antisense 15μl
Procedure
Please see protocol3
Experiment:Restriction enzyme digestion
member
Same above
Materials
- PCR production
- m buffer 30μl
- BSA 30μl
- Xba1 6μl
- Spe1 6μl
Procedure
Please see protocol4
Experiment:Transformation
member
Same above
Materials , Procedure
Please refer to protocol9
