"
Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/23
From 2010.igem.org
(Difference between revisions)
(New page: {{:Team:Tokyo_Metropolitan/Header}} ==2010/8/23 Monday (watachin)== ===Experiment:Direct PCR(K12-bcsA,K12-bcsC)=== '''Member'''<br /> Bambi75 and watachin '''Materials''' *E.coli K12 col...) |
|||
| Line 1: | Line 1: | ||
| - | {{:Team:Tokyo_Metropolitan/ | + | {{:Team:Tokyo_Metropolitan/Notebook/Fiber}} |
==2010/8/23 Monday (watachin)== | ==2010/8/23 Monday (watachin)== | ||
| Line 19: | Line 19: | ||
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">the protocol</a></html> | follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">the protocol</a></html> | ||
| - | |||
| Line 28: | Line 27: | ||
'''Material''' | '''Material''' | ||
*LB media 200ml | *LB media 200ml | ||
| - | + | *Distilled water 200ml | |
| - | + | *LB Broth 4g | |
*ampicillin (50mg/ml) 200μl | *ampicillin (50mg/ml) 200μl | ||
Revision as of 18:27, 22 October 2010
E.coli Fiber Project Notebook
|
|
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Contents |
2010/8/23 Monday (watachin)
Experiment:Direct PCR(K12-bcsA,K12-bcsC)
Member
Bambi75 and watachin
Materials
- E.coli K12 colony
- 10µM/l forward Primer 5µl(K12-bcsA,K12-bcsC)
- 10µM/l reverse Primer 5µl(K12-bcsA,K12-bcsC)
- 10×EX taq buffer 10µl
- 2.5mM each dNTP mixture 10µl
- EX taq polymerase 1µl
- milli-Q 71µl
Procedure
follow the protocol
Experiment:Making media(LB broth + amp)
Member
Bambi75 and watachin
Material
- LB media 200ml
- Distilled water 200ml
- LB Broth 4g
- ampicillin (50mg/ml) 200μl
Experiment:Restriction enzyme digestion
Member
