Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/23

From 2010.igem.org

(Difference between revisions)
(Experiment:Colony PCR)
(Experiment:Colony PCR)
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**bcsD:65~96
**bcsD:65~96
other materials were same as <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
other materials were same as <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
 +
 +
'''procedure'''
 +
 +
see html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
 +
'''result'''
'''result'''

Revision as of 20:57, 24 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

2010/10/23 Saturday (Naoto)

member

naoto and watachin

Experiment:Colony PCR

material

  • colony of E.coli
    • bcsB:1~32
    • bcsC:33~64
    • bcsD:65~96

other materials were same as protocol3

procedure

see html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>


result

From the length of bands

bcsC→No.54 and 64

bcsD→No.75

seem to be correct inserts