Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/14

From 2010.igem.org

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{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
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__NOTOC__
==2010/10/14 Thursday (Naoto)==
==2010/10/14 Thursday (Naoto)==
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'''member'''
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:'''member'''
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naoto,maki and watachin  
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:naoto,maki and watachin  
===Preparation:Measure DNA concentration===
===Preparation:Measure DNA concentration===
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'''Material'''
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:'''Material'''
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*bcsA
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:*bcsA
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*bcsB
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:*bcsB
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*bcsC
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:*bcsC
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*bcsD
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:*bcsD
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*pSB1C3
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:*pSB1C3
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*TE buffer
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:*TE buffer
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'''Procedure'''
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:'''Procedure'''
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#mix 4μl of DNA and 4μl of TE buffer
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:#mix 4μl of DNA and 4μl of TE buffer
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#set mixture in absorption spectrometer
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:#set mixture in absorption spectrometer
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#measure DNA concentrations
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:#measure DNA concentrations
===Experiment:PCR===
===Experiment:PCR===
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'''material'''
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:'''material'''
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*templete DNA (from A.xylinum and pSB1C3)
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:*templete DNA (from A.xylinum and pSB1C3)
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you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol3</a></html>
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:you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol3</a></html>
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'''procedure'''
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:'''procedure'''
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see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol3</a></html>
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:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol3</a></html>
===Experiment:Electrophoresis===
===Experiment:Electrophoresis===
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'''material'''
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:'''material'''
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*bcsA  
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:*bcsA  
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*bcsB
+
:*bcsB
-
*bcsC
+
:*bcsC
-
*bcsD
+
:*bcsD
-
you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
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:*pSB1C3
 +
:you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
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'''procedure'''
+
:'''procedure'''
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refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
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:refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
<br/>
<br/>

Latest revision as of 17:57, 27 October 2010


E.coli Fiber Project Notebook

August 2010
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September 2010
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October 2010
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2010/10/14 Thursday (Naoto)

member
naoto,maki and watachin

Preparation:Measure DNA concentration

Material
  • bcsA
  • bcsB
  • bcsC
  • bcsD
  • pSB1C3
  • TE buffer
Procedure
  1. mix 4μl of DNA and 4μl of TE buffer
  2. set mixture in absorption spectrometer
  3. measure DNA concentrations

Experiment:PCR

material
  • templete DNA (from A.xylinum and pSB1C3)
you can see other materials at protocol3
procedure
see protocol3

Experiment:Electrophoresis

material
  • bcsA
  • bcsB
  • bcsC
  • bcsD
  • pSB1C3
you can see materials at protocol4
procedure
refer to protocol4

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Fiber/2010/10/14"