Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/14

From 2010.igem.org

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{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
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__NOTOC__
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==2010/10/14 Thursday (Naoto)==
 +
:'''member'''
 +
 +
:naoto,maki and watachin
 +
 +
===Preparation:Measure DNA concentration===
 +
:'''Material'''
 +
:*bcsA
 +
:*bcsB
 +
:*bcsC
 +
:*bcsD
 +
:*pSB1C3
 +
:*TE buffer
 +
 +
:'''Procedure'''
 +
:#mix 4μl of DNA and 4μl of TE buffer
 +
:#set mixture in absorption spectrometer
 +
:#measure DNA concentrations
 +
 +
===Experiment:PCR===
 +
:'''material'''
 +
:*templete DNA (from A.xylinum and pSB1C3)
 +
:you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol3</a></html>
 +
 +
:'''procedure'''
 +
 +
:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol3</a></html>
 +
 +
===Experiment:Electrophoresis===
 +
:'''material'''
 +
:*bcsA
 +
:*bcsB
 +
:*bcsC
 +
:*bcsD
 +
:*pSB1C3
 +
:you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
 +
 +
:'''procedure'''
 +
 +
:refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
 +
 +
<br/>

Latest revision as of 17:57, 27 October 2010


E.coli Fiber Project Notebook

August 2010
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September 2010
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October 2010
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2010/10/14 Thursday (Naoto)

member
naoto,maki and watachin

Preparation:Measure DNA concentration

Material
  • bcsA
  • bcsB
  • bcsC
  • bcsD
  • pSB1C3
  • TE buffer
Procedure
  1. mix 4μl of DNA and 4μl of TE buffer
  2. set mixture in absorption spectrometer
  3. measure DNA concentrations

Experiment:PCR

material
  • templete DNA (from A.xylinum and pSB1C3)
you can see other materials at protocol3
procedure
see protocol3

Experiment:Electrophoresis

material
  • bcsA
  • bcsB
  • bcsC
  • bcsD
  • pSB1C3
you can see materials at protocol4
procedure
refer to protocol4

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Fiber/2010/10/14"