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Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/03
From 2010.igem.org
(Difference between revisions)
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==2010/10/03 Sunday (Naoto)== | ==2010/10/03 Sunday (Naoto)== | ||
===Experiment:PCR of bcsA('''A.xylinum''')=== | ===Experiment:PCR of bcsA('''A.xylinum''')=== | ||
| - | '''member''' | + | :'''member''' |
| - | naoto and watachin | + | :naoto and watachin |
| - | '''marerial''' | + | :'''marerial''' |
| - | *bcsA(''A.xylinum'') | + | :*bcsA(''A.xylinum'') |
| - | If you want to know other materials, see protocol3 | + | :If you want to know other materials, see protocol3 |
| - | '''procedure''' | + | :'''procedure''' |
| - | see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html> | + | :see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html> |
===Experiment:Electrophoresis of bcsC,D('''A.xylinum'''),bcsA,B,C,EFG,Q('''E.coli'''),and pSB13=== | ===Experiment:Electrophoresis of bcsC,D('''A.xylinum'''),bcsA,B,C,EFG,Q('''E.coli'''),and pSB13=== | ||
| - | '''member''' | + | :'''member''' |
| - | naoto and watachin | + | :naoto and watachin |
| - | '''marerial''' | + | :'''marerial''' |
| - | *bcsC,D(''A.xylinum''),bcsA,B,C,EFG,Q(''E.coli''),pSB13 | + | :*bcsC,D(''A.xylinum''),bcsA,B,C,EFG,Q(''E.coli''),pSB13 |
| - | If you want to know other materials, see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html> | + | :If you want to know other materials, see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html> |
| - | '''procedure''' | + | :'''procedure''' |
| - | see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html> | + | :see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html> |
===Experiment:Miniprep of BBa_K145001=== | ===Experiment:Miniprep of BBa_K145001=== | ||
| - | '''member''' | + | :'''member''' |
| - | naoto and watachin | + | :naoto and watachin |
| - | '''marerial''' | + | :'''marerial''' |
| - | *Preculture of ''E.coli'' having BBa_K145001 | + | :*Preculture of ''E.coli'' having BBa_K145001 |
| - | If you want to know other materials, see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol9</a></html> | + | :If you want to know other materials, see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol9</a></html> |
| - | '''procedure''' | + | :'''procedure''' |
| - | see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol9</a></html> | + | :see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol9</a></html> |
===Experiment:Ligation of pSB1C3 and bcsC,D(''A.xylinum''),bcsA(''E.coli'')=== | ===Experiment:Ligation of pSB1C3 and bcsC,D(''A.xylinum''),bcsA(''E.coli'')=== | ||
| - | '''member''' | + | :'''member''' |
| - | naoto and watachin | + | :naoto and watachin |
| - | '''marerial''' | + | :'''marerial''' |
| - | *pSB1C3 | + | :*pSB1C3 |
| - | *bcsC,D(''A.xylinum''),bcsA(''E.coli'') | + | :*bcsC,D(''A.xylinum''),bcsA(''E.coli'') |
| - | If you want to know other materials, see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol7</a></html> | + | :If you want to know other materials, see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol7</a></html> |
| - | '''procedure''' | + | :'''procedure''' |
| - | see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html> | + | :see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html> |
===Experiment:Transformation of parts of bcsC,D(''A.xylinum''),bcsA(''E.coli'')=== | ===Experiment:Transformation of parts of bcsC,D(''A.xylinum''),bcsA(''E.coli'')=== | ||
| - | '''member''' | + | :'''member''' |
| - | naoto and watachin | + | :naoto and watachin |
| - | '''marerial''' | + | :'''marerial''' |
| - | * parts of bcsC,D(''A.xylinum''),bcsA(''E.coli'') | + | :* parts of bcsC,D(''A.xylinum''),bcsA(''E.coli'') |
| - | If you want to know other materials, see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html> | + | :If you want to know other materials, see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html> |
| - | '''procedure''' | + | :'''procedure''' |
| - | see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html> | + | :see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html> |
===Experiment:Electrophoresis of bcsA('''A.xylinum''')=== | ===Experiment:Electrophoresis of bcsA('''A.xylinum''')=== | ||
| - | '''member''' | + | :'''member''' |
| - | naoto and watachin | + | :naoto and watachin |
| - | '''marerial''' | + | :'''marerial''' |
| - | *bcsA(''A.xylinum'') | + | :*bcsA(''A.xylinum'') |
| - | If you want to know other materials, see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html> | + | :If you want to know other materials, see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html> |
| - | '''procedure''' | + | :'''procedure''' |
| - | see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html> | + | :see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html> |
| - | '''result''' | + | :'''result''' |
| - | Band of bcsA could be seen clearly | + | :Band of bcsA could be seen clearly |
===Experiment:Purification of bcsA('''A.xylinum''')=== | ===Experiment:Purification of bcsA('''A.xylinum''')=== | ||
| - | '''member''' | + | :'''member''' |
| - | naoto and watachin | + | :naoto and watachin |
| - | '''marerial''' | + | :'''marerial''' |
| - | *bcsA(''A.xylinum'') in agarose gel | + | :*bcsA(''A.xylinum'') in agarose gel |
| - | If you want to know other materials, see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5</a></html> | + | :If you want to know other materials, see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5</a></html> |
| - | '''procedure''' | + | :'''procedure''' |
| - | see protocol4 <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html> | + | :see protocol4 <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html> |
Latest revision as of 16:42, 27 October 2010
E.coli Fiber Project Notebook
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2010/10/03 Sunday (Naoto)
Experiment:PCR of bcsA(A.xylinum)
- member
- naoto and watachin
- marerial
- bcsA(A.xylinum)
- If you want to know other materials, see protocol3
- procedure
- see protocol3
Experiment:Electrophoresis of bcsC,D(A.xylinum),bcsA,B,C,EFG,Q(E.coli),and pSB13
- member
- naoto and watachin
- marerial
- bcsC,D(A.xylinum),bcsA,B,C,EFG,Q(E.coli),pSB13
- If you want to know other materials, see protocol4
- procedure
- see protocol4
Experiment:Miniprep of BBa_K145001
- member
- naoto and watachin
- marerial
- Preculture of E.coli having BBa_K145001
- If you want to know other materials, see protocol9
- procedure
- see protocol9
Experiment:Ligation of pSB1C3 and bcsC,D(A.xylinum),bcsA(E.coli)
- member
- naoto and watachin
- marerial
- pSB1C3
- bcsC,D(A.xylinum),bcsA(E.coli)
- If you want to know other materials, see protocol7
- procedure
- see protocol4
Experiment:Transformation of parts of bcsC,D(A.xylinum),bcsA(E.coli)
- member
- naoto and watachin
- marerial
- parts of bcsC,D(A.xylinum),bcsA(E.coli)
- If you want to know other materials, see protocol8
- procedure
- see protocol8
Experiment:Electrophoresis of bcsA(A.xylinum)
- member
- naoto and watachin
- marerial
- bcsA(A.xylinum)
- If you want to know other materials, see protocol4
- procedure
- see protocol4
- result
- Band of bcsA could be seen clearly
Experiment:Purification of bcsA(A.xylinum)
- member
- naoto and watachin
- marerial
- bcsA(A.xylinum) in agarose gel
- If you want to know other materials, see protocol5
- procedure
- see protocol4 protocol4
