Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/27

From 2010.igem.org

(Difference between revisions)

Latest revision as of 16:55, 27 October 2010

E.coli Fiber Project Notebook

2010/9/27 Monday (watachin)(bambi75)

Experiment:PCR/bcsA and D(A.xylium)/bcsA,B and C(E.coli)

Member

watachin and bambi75

Materials,Procedure

Same on August 23th.

95℃5min
95℃30sec
55℃30sec
72℃4min
72℃5min
10℃∞

（95℃～72℃/30cycle）

see protocol3

Experiment:Agarose Gels Electrophoresis

Member

Same above

Materials and Procedure

Same protocol the protocol4

PCR Product of insert is the above things.

Experiment:Restriction enzyme digestion

Member

Same above

Materials and Procedure

Same protocol the protocol6

Experiment:Agarose gel Electrophoresis

Member

Same above

Materials and Procedure

Same protocol4

PCR Production is the above things.

Experiment:Ligation

Member

Same above

Materials and Procedure

Follow protocol8(Ligation).

bcsB(E.coli)
bcsC(E.coli)

Experiment:Ligation and Transformation

Member

Same above

Materials and Procedure

Same protocol on September 21st.

Experimet:PCR/bcsQ and bcsEFG(E.coli)

Member

watachin and bambi75

Materials,Procedure

Same on August 23th.

95℃5min
95℃30sec
55℃30sec
72℃4min
72℃5min
10℃∞

（95℃～72℃/30cycle）

Experiment:Agarose Gels Electrophoresis

Member

Same above

Materials and Procedure

Same protocol on August 16th.

PCR Product of insert is the above things.

Experiment:PCR

Member

watachin and bambi75

Materials,Procedure

PCR of A.xylinum bcsA,D and E.coli bcsA,B,C ,pSB1C3

Same on August 23th.

95℃5min
95℃30sec
55℃30sec
72℃4min
72℃5min
10℃∞

（95℃～72℃/30cycle）

see protocol3

Experiment:Electrophoreses of PCR production

Member

Same above

Materials and Procedure

Same protocol on August 16th.

PCR Product of insert is the above things.

Result

Experiment:Digestion

bcsA → XbaI,SpeI

bcsB → XbaI

bcsC → SpeI

pSB1C3 → XbaI, SpeI

Experiment:Electrophoreses of PCR productions

Member

Same above

Materials and Procedure

Same protocol on August 16th.

PCR Product of insert is the above things.

Result

Experiment:DNA Purification

member

naoto and watachin

procedure

see protocol6

Experiment:Ligation

Member

Same above

Materials and Procedure

Follow protocol8(Ligation).

Experiment:DNA Purification

member

naoto and watachin

procedure

see protocol6

Experiment:Ligation

Member

Same above

Materials and Procedure

Follow protocol8(Ligation).

Experiment:Transfomation

Member

watachin

Materials,Procedure

see protocol9

Experiment:PCR

Member

watachin

Materials,Procedure

Same on August 23th.

95℃5min
95℃30sec
55℃30sec
72℃4min
72℃5min
10℃∞

（95℃～72℃/30cycle）

see protocol3

Experiment:Electrophoreses of PCR productions

Member

Same above

Materials and Procedure

Same protocol on August 16th.

PCR Product of insert is the above things.

Result

@@ Line 1: / Line 1: @@
-{{:Team:Tokyo_Metropolitan/Header}}
+{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
+__NOTOC__
+==2010/9/27 Monday (watachin)(bambi75)==
+===Experiment:PCR/bcsA and D(A.xylium)/bcsA,B and C(E.coli)===
-==2010/9/27 Monday (watachin)==
+:'''Member'''
-===PCR===
+:watachin and bambi75
-PCR of A.xylinum bcsA,D and E.coli bcsA,B,C ,pSB1C3
-===Electrophoreses of PCR production===
-'''Result'''
-[[Image:2010-09-27-01.jpg]]
+:'''Materials,Procedure'''
+:Same on August 23th.
+:*95℃5min
+:*95℃30sec
+:*55℃30sec
+:*72℃4min
+:*72℃5min
+:*10℃∞
+:（95℃～72℃/30cycle）
-===Digestion===
+:see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR protocol3]
-bcsA → Xba,Spe
-bcsB → Xba
+===Experiment:Agarose Gels Electrophoresis===
-bcsC → Spe
+:'''Member'''
-pSB1C3 → Xba, Spe
+:Same above
-===Electrophoreses of PCR productions===
+:'''Materials and Procedure'''
-'''Result'''
+:Same protocol <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80agarose gel electrophoresis">the protocol4</a></html>
-[[Image:2010-09-27-02.jpg]]
+:PCR Product of insert is the above things.
+===Experiment:Restriction enzyme digestion===
-===DNA Purification===
+:'''Member'''
-===Ligation===
+:Same above
-===DNA Purification===
+:'''Materials and Procedure'''
-===Ligation===
+:Same protocol <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80restriction enzyme digestion">the protocol6</a></html>
-===Transfomation===
+===Experiment:Agarose gel Electrophoresis===
-===PCR===
+:'''Member'''
-===Electrophoreses of PCR productions===
+:Same above
+:'''Materials and Procedure'''
+:Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
+:PCR Production is the above things.
+===Experiment:Ligation===
+:'''Member'''
+:Same above
+:'''Materials and Procedure'''
+:Follow [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol8:Ligation protocol8(Ligation)].
+:*bcsB(E.coli)
+:*bcsC(E.coli)
+===Experiment:Ligation and Transformation===
+:'''Member'''
+:Same above
+:'''Materials and Procedure'''
+:Same protocol on September 21st.
+===Experimet:PCR/bcsQ and bcsEFG(E.coli)===
+:'''Member'''
+:watachin and bambi75
+:'''Materials,Procedure'''
+:Same on August 23th.
+:*95℃5min
+:*95℃30sec
+:*55℃30sec
+:*72℃4min
+:*72℃5min
+:*10℃∞
+:（95℃～72℃/30cycle）
+===Experiment:Agarose Gels Electrophoresis===
+:'''Member'''
+:Same above
+:'''Materials and Procedure'''
+:Same protocol on August 16th.
+:PCR Product of insert is the above things.
+===Experiment:PCR===
+:'''Member'''
+:watachin and bambi75
+:'''Materials,Procedure'''
+:PCR of A.xylinum bcsA,D and E.coli bcsA,B,C ,pSB1C3
+:Same on August 23th.
+:*95℃5min
+:*95℃30sec
+:*55℃30sec
+:*72℃4min
+:*72℃5min
+:*10℃∞
+:（95℃～72℃/30cycle）
+:see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR protocol3]
+===Experiment:Electrophoreses of PCR production===
+:'''Member'''
+:Same above
+:'''Materials and Procedure'''
+:Same protocol on August 16th.
+:PCR Product of insert is the above things.
+:'''Result'''
+:[[Image:2010-09-27-01.jpg]]
+===Experiment:Digestion===
+:bcsA → XbaI,SpeI
+:bcsB → XbaI
+:bcsC → SpeI
+:pSB1C3 → XbaI, SpeI
+===Experiment:Electrophoreses of PCR productions===
+:'''Member'''
+:Same above
+:'''Materials and Procedure'''
+:Same protocol on August 16th.
+:PCR Product of insert is the above things.
+:'''Result'''
+:[[Image:2010-09-27-02.jpg]]
+===Experiment:DNA Purification===
+:'''member'''
+:naoto and watachin
+:'''procedure'''
+:see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol6:DNA_Purification_with_silica_gel protocol6]
+===Experiment:Ligation===
+:'''Member'''
+:Same above
+:'''Materials and Procedure'''
+:Follow [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol8:Ligation protocol8(Ligation)].
+===Experiment:DNA Purification===
+:'''member'''
+:naoto and watachin
+:'''procedure'''
+:see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol6:DNA_Purification_with_silica_gel protocol6]
+===Experiment:Ligation===
+:'''Member'''
+:Same above
+:'''Materials and Procedure'''
+:Follow [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol8:Ligation protocol8(Ligation)].
+===Experiment:Transfomation===
+:'''Member'''
+:watachin
+:'''Materials,Procedure'''
+:see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol9:Transformation protocol9]
+===Experiment:PCR===
+:'''Member'''
+:watachin
+:'''Materials,Procedure'''
+:Same on August 23th.
+:*95℃5min
+:*95℃30sec
+:*55℃30sec
+:*72℃4min
+:*72℃5min
+:*10℃∞
+:（95℃～72℃/30cycle）
+:see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR protocol3]
+===Experiment:Electrophoreses of PCR productions===
+:'''Member'''
+:Same above
+:'''Materials and Procedure'''
+:Same protocol on August 16th.
+:PCR Product of insert is the above things.
 '''Result'''
-[[Image:2010-09-27-03.jpg]]
+:[[Image:2010-09-27-03.jpg]]
+<br/>