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Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/25
From 2010.igem.org
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| - | :See <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol# | + | :See <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol6:DNA_Purification_with_silica_gel">protocol5-2</a></html> |
===Experiment:Ligatiom=== | ===Experiment:Ligatiom=== | ||
Revision as of 14:56, 27 October 2010
E.coli Fiber Project Notebook
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2010/09/25 Saturday(NEX)
Experiment:PCR
- Member
- easily
- Material
- templete DNA:pSB1C3
- sense primers of pSB1C3
- antisense primers of pSB1C3
- Procedure
- Refer to protocol3
Experiment:Ligatiom
- Member
- easily
- Material
- bcsA from E.coli 20μl
- bcsB from E.coli 20μl
- 2×ligation mix 40μl
- Procedure
- Follow to protocol7
Experiment:Agarose gel electrophoresis
- Member
- easily
- Material
- Today's PCR productions
- Today's ligation productions
- Agarose gel
- Procedure
- Refer to protocol4
- Result
- pSB1C3 were appeared
Experiment:DNA purification with silica gel
- Member
- easily
- Materials
- pSB1C3
- Procedure
- See protocol5-2
Experiment:Ligatiom
- Member
- easily
- Material
- pSB1C3 3μl
- bcsA,B,C,D from A.xylinum or bcsA from E.coli 3μl
- 2×ligation mix 6μl
- Procedure
- Follow to protocol7
