Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/10

From 2010.igem.org

(Difference between revisions)
 
Line 4: Line 4:
===Experiment:PCR===
===Experiment:PCR===
-
'''Member'''<br/>
+
:'''Member'''<br/>
-
naoto, easily
+
:naoto, easily
-
'''Material'''<br/>
+
:'''Material'''<br/>
-
*sense primers of A.xylinum bcsA,B,C,D and E.coli bcsB
+
:*sense primers of A.xylinum bcsA,B,C,D  
-
*antisense primers of A.xylinum bcsA,B,C,D and E.coli bcsB
+
:*antisense primers of A.xylinum bcsA,B,C,D
-
 
+
-
'''Procedure'''<br/>
+
-
Refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR">protocol3</a></html>
+
 +
:'''Procedure'''<br/>
 +
:Refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR">protocol3</a></html>
===Experiment:Agarose gel electrophoresis===
===Experiment:Agarose gel electrophoresis===
-
'''Member'''<br />
+
:'''Member'''<br />
-
Same above
+
:Same above
-
'''Material'''
+
:'''Material'''
-
*PCR productions
+
:*PCR productions
-
*Agarose gel
+
:*Agarose gel
-
'''Procedure'''<br/>
+
:'''Procedure'''<br/>
-
Refer to <html><a href="Agarose gel electrophoresis">protocol4</a></html>
+
:Refer to <html><a href="Agarose gel electrophoresis">protocol4</a></html>
-
'''Result'''
+
:'''Result'''
[[Image:100910.jpg]]
[[Image:100910.jpg]]
-
 
===Experiment:DNA purification from agarose gel===
===Experiment:DNA purification from agarose gel===
-
'''Member'''<br />
+
:'''Member'''<br />
-
Same above
+
:Same above
-
'''Materials'''<br />
+
:'''Materials'''<br />
-
*Agarose gel with bcsD from A.xylinum
+
:*bcsD in agarose gel
-
'''Procedure'''<br />
+
:'''Procedure'''<br />
-
See <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol5-1:DNA_purification_from_agarose_gel">protocol5-1</a></html>
+
:See <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol5:DNA_purification_from_agarose_gel">protocol5</a></html>

Latest revision as of 06:43, 27 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

2010/09/10 Friday(NEX)

Experiment:PCR

Member
naoto, easily
Material
  • sense primers of A.xylinum bcsA,B,C,D
  • antisense primers of A.xylinum bcsA,B,C,D
Procedure
Refer to protocol3

Experiment:Agarose gel electrophoresis

Member
Same above
Material
  • PCR productions
  • Agarose gel
Procedure
Refer to protocol4
Result

100910.jpg

Experiment:DNA purification from agarose gel

Member
Same above
Materials
  • bcsD in agarose gel
Procedure
See protocol5
Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Fiber/2010/09/10"