Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/13

(Difference between revisions)

Latest revision as of 21:28, 26 October 2010

E.coli Fiber Project Notebook

Member

NEX , bambi75 and watachin

Materials

Procedure

mix materials : M buffer(5μl) , BSA1(5μl) , Xba1(1μl) and Spe1 (1μl) to PCR products(50μl). Repeat this process 3 times , for 3 PCR products.
incubation(37℃).

@@ Line 3: / Line 3: @@
 ==2010/08/13 Friday (bambi75)==
-===Experiment:Digestion of bcs===
+===Experiment:Restrict enzyme digestion===
-'''member'''
+:'''Member'''
-NEX , bambi75 and watachin
+:NEX , bambi75 and watachin
+:'''Materials'''
+:*bcsA,B,C,D(each):50μl
+:*10×M buffer:15μl
+:*BSA:15μl
+:*XbaI:3μl
+:*SpeI:3μl
-'''Materials'''
+:'''Procedure'''
-*PCR products  50μl×3
+:#mix materials : M buffer(5μl) , BSA1(5μl) , Xba1(1μl) and Spe1 (1μl) to PCR products(50μl). Repeat this process 3 times , for 3 PCR products.
-*m buffer  15μl
+:#incubation(37℃).
-*BSA 15μl
-*Xba1  3μl
-*Spe1  3μl
-'''Procedure'''
-#mix materials : m buffer(5μl) , BSA1(5μl) , Xba1(1μl) and Spe1 (1μl) to PCR products(50μl). Repeat this process 3 times , for 3 PCR products.
-#incubation(37℃).
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