Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/11

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Latest revision as of 21:25, 26 October 2010

E.coli Fiber Project Notebook

2010/08/11 Wednesday (Naoto)

Experiment:Grow up a culture of A.xylinum

Member

easily and naoto

Material

A.xylinum JCM 7644 strain
Acetobacter medium (see protocol1)
ｒecovery solution for A.xylinum

Procedure

refer to protocol1

Experiment:Grow up a culture of E.coli

Member

NEX,Bambi75 and watachin

Material

E.coli K12 JM109
LB medium (see protocol2)

Procedure

refer to protocol2

@@ Line 1: / Line 1: @@
-{{:Team:Tokyo_Metropolitan/Header}}
+{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
-==2010/8/11 Wednesday (Naoto)==
+==2010/08/11 Wednesday (Naoto)==
-===Experiment:Grow up a culture of ''A.xylinum''===
+===Experiment:Grow up a culture of A.xylinum===
-'''Member'''<br />
+:'''Member'''
-easily and naoto
-'''Materials'''
+:easily and naoto
-*''A.xylinum'' JCM strain 7644
+:'''Material'''
-*media for A.xylinum(Open Wet Ware recommended)
-*media for A.xylinum(JCM recommended) 100ml
- glucoce 10g
- yeast extract 1g
- CaCO3 3g
- DW 100ml
- (add agar 1.5g for plates)
-*ｒecovery solution for ''A.xylinum'' 100ml
- peptone 0.5g
- yeast extract 0.3g
- DW 100ml
-'''Procedure'''
+:*''A.xylinum'' JCM 7644 strain
+:*Acetobacter medium (see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Grow_up_a_culture_of_A.xylinum">protocol1</a></html>)
+:*ｒecovery solution for A.xylinum
+:'''Procedure'''
-#prepared media (plates of Open Wet Ware recommended media were made yesterday)
+:refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Grow_up_a_culture_of_A.xylinum">protocol1</a></html>
-#autoclaved to sterilize media(121°C　20minute)
-#added ｒecovery solution to an ampule which had ''A.xylinum''
-#streaked ''A.xylinum'' onto plates or in media
-#incubated at 26°C
-===Experiment:Grow up a culture of ''E.coli''===
+===Experiment:Grow up a culture of E.coli===
-'''Member'''<br />
+:'''Member'''
-NEX,Bambi75 and watachin
-'''Materials'''<br />
+:NEX,Bambi75 and watachin
-LB plates
-'''Procedure'''
+:'''Material'''
-#streaked ''E.coli'' onto plates
+:*''E.coli'' K12 JM109
-#incubated at 37°C
+:*LB medium (see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Grow_up_a_culture_of_E.coli">protocol2</a></html>)
+:'''Procedure'''
+:refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Grow_up_a_culture_of_E.coli">protocol2</a></html>
+<br/>