Team:Panama/5 August 2010
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==='''August 5'''=== | ==='''August 5'''=== | ||
- | DNA concentration measurements | + | ==Plasmid DNA concentration measurements== |
1.'''Promoter:''' 27,8 ng/ul | 1.'''Promoter:''' 27,8 ng/ul | ||
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+ | This reaction was incubated at 37ºC for 3 hours, in the thermocycler. | ||
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'''NOTE:''' The plasmid was not digested because we didn't resuspend it (it comes in a lyophilized form). | '''NOTE:''' The plasmid was not digested because we didn't resuspend it (it comes in a lyophilized form). |
Revision as of 04:45, 27 October 2010
August 5
Plasmid DNA concentration measurements
1.Promoter: 27,8 ng/ul
2.RBS: 42 ng/ul
3.Reporter: 40,3 ng/ul
Digestion mix Promoter and RBS:
This reaction was incubated at 37ºC for 3 hours, in the thermocycler.
NOTE: The plasmid was not digested because we didn't resuspend it (it comes in a lyophilized form).
Electrophoresis of the terminator BioBrick.
We prepared 1% of agarose. In each well we add: 5ul of sample, 2 ul of loading buffer. The marker used is HindIII, 1ul + 2ul loading buffer.
Now, for some reason, the sample didn't stay inside the wells.
[[Image:|350px|thumb|left|alt text]]
The expected band was 2160pb but actually it resulted much higher, aproximately in the 3000pb range and seems too high. This isn´t a satisfactory result because it doesn't have the necessary length.
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