Team:Panama/13 August 2010

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iGEM Panama

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August 13

Digestion Reaction

Today we did the digest the plasmid that contain the promoter with the EcoR1 and Spe1 enzymes, and the plasmid with the RBS was digested with the Xha1 and Pst1 enzymes.

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RESULTS

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PCR Rhlab amplification


Now that we have our Rh1ab we are going to run a PCR of it using our first set of primers.

RhT-F1a 5' GCGATAGCTGTTTGCCTGTT 3'

RhT-R2a 5' TGGCAACCCTATCTGTTATGC 3'

First of all, we have to resuspend our primers since they come in a lyophilized manner. We need a stock solution of 100uM. Our working solution has a concentration of 10uM.

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RESULTS



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We tested three concentrations of primers to see which one worked better for us and we can see that all three concentrations worked! So from now on, just to be on the safe side, we're going to use the concentration of 1uM.

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