Team:Panama/5 August 2010

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iGEM Panama

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August 5

Plasmid DNA concentration measurements

1.Promoter: 27,8 ng/ul

2.RBS: 42 ng/ul

3.Reporter: 40,3 ng/ul

Digestion mix Promoter and RBS:

After the plasmid DNA concentration measurements we continued with the restriction reaction of the plasmids.

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This reaction was incubated at 37ºC for 3 hours, in the thermocycler.








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NOTE: The plasmid was not digested because we didn't resuspend it in water (it comes in a lyophilized form).

Electrophoresis of the terminator BioBrick.

We prepared 1% of agarose. In each well we add: 5ul of sample, 2 ul of loading buffer. The marker used is HindIII, 1ul + 2ul loading buffer.

Now, for some reason, the sample didn't stay inside the wells.

[[Image:|350px|thumb|left|alt text]]






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The expected band was 2160pb but actually it resulted much higher, aproximately in the 3000pb range and seems too high. This isn´t a satisfactory result because it doesn't have the necessary length.

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