Team:Panama/5 August 2010
From 2010.igem.org
August 5
Plasmid DNA concentration measurements
1.Promoter: 27,8 ng/ul
2.RBS: 42 ng/ul
3.Reporter: 40,3 ng/ul
Digestion mix Promoter and RBS:
After the plasmid DNA concentration measurements we continued with the restriction reaction of the plasmids.
This reaction was incubated at 37ºC for 3 hours, in the thermocycler.
NOTE: The plasmid was not digested because we didn't resuspend it in water (it comes in a lyophilized form).
Electrophoresis of the terminator BioBrick.
We prepared 1% of agarose. In each well we add: 5ul of sample, 2 ul of loading buffer. The marker used is HindIII, 1ul + 2ul loading buffer.
Now, for some reason, the sample didn't stay inside the wells.
[[Image:|350px|thumb|left|alt text]]
The expected band was 2160pb but actually it resulted much higher, aproximately in the 3000pb range and seems too high. This isn´t a satisfactory result because it doesn't have the necessary length.
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