Team:Panama/11 October 2010
From 2010.igem.org
October 11
Today we measure the concentration of the purified PCR product and the result was 107ug/ml.
We also determined that for the cloning using the pGEMT Easy Kit it is necessary to use a ratio of 3:1. 3 being the vector amount and 1 being the insert amount.
Now we are cloning with the pGEMT Easy Kit
Electrophoresis of the ligation product: Terminator, GFP
1% agarose gel, TBE 1X, 2.5ul EtBr. We decided to leave well 3 empty just so it'd look pretty, pretty and it does!
3055 PsB1AC3
151 Terminator
742 GFP
3948 bp
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