Team:Panama/23 August 2010
From 2010.igem.org
August 23
After all of this, we finally, started the ligation of RBS, promoter and plasmid.
The ligation mix that we'll use:
So to get a final volume of 20ul, then we incubated this for 2 hours at room temperature and later at 80ÂșC for 20 minutes to deactivate the enzyme.
And good news for everyone, the ligation was done right! So now we have our RBS and promotor in a plasmid.
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