Team:Panama/22 September 2010

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iGEM Panama

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September 22

Digestion of terminator (This has nothing to do with Arnold's peristaltic movements)

Today we did the plasmid terminator digestion with the Xba1 and Pst1 enzyme.

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3 hours at 37°C and 20 minutes at 80°C disactivation.


Agarose Gel 1%

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Size of insert: 151 bp

Size of Plasmid: 3168 bp


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