Team:Panama/5 August 2010
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'''NOTE:''' The plasmid was not digested because we didn't resuspend it (it comes in a lyophilized form). | '''NOTE:''' The plasmid was not digested because we didn't resuspend it (it comes in a lyophilized form). | ||
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'''Electrophoresis of the terminator BioBrick'''. | '''Electrophoresis of the terminator BioBrick'''. | ||
Revision as of 18:40, 25 October 2010
August 5
DNA concentration measurements
1.Promoter: 27,8 ng/ul
2.RBS: 42 ng/ul
3.Reporter: 40,3 ng/ul
Digestion mix Promoter and RBS:
3 hours incubation at 37ºC, in the thermocycler.
NOTE: The plasmid was not digested because we didn't resuspend it (it comes in a lyophilized form).
Electrophoresis of the terminator BioBrick.
We prepared 1% of agarose. In each well we add: 5ul of sample, 2 ul of loading buffer.
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