Team:Panama/29 July 2010

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The bands that were obtained corresponded to the expected results and this proves the success of the extraction.
The bands that were obtained corresponded to the expected results and this proves the success of the extraction.
{{:Team:Panama/calendar2}}
{{:Team:Panama/calendar2}}

Revision as of 20:37, 26 October 2010

iGEM Panama

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July 29

We performed the E. coli transformation again, with three BioBrick parts. In order to check the success of the extraction, we made an electrophresis in a 1% agarose gel.

Electrophoresis

70mL of TBE 1X

0,7g of agarose

In each well, we added 2ul of loading buffer and 5ul of the sample. Final volume 7ul.

Agarose gel wells:

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The bands that were obtained corresponded to the expected results and this proves the success of the extraction.

May
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
June
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
July
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
August
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31
September
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
October
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31