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Team:Panama/28 July 2010
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4% 3:1 Agarose (Amresco, Cat no: 9012-36-6) | 4% 3:1 Agarose (Amresco, Cat no: 9012-36-6) | ||
| - | + | 100ml TBE 1X. | |
4g of agarose | 4g of agarose | ||
| - | + | ||
| - | + | The agarose is disolved in the TBE using heat. At first, the mixture is opaque but as the agarose disolves, the mixture becomes clear. At this point we add 5ul of Ethydium bromide. This can then be poured into the casting chamber. | |
| + | |||
Agarose gel: | Agarose gel: | ||
| - | + | ||
| + | Once the gel was set, in each well we added 20 ul of sample, plus 2ul of loading buffer. Total volume = 22ul. | ||
| + | |||
| + | The molecular weight marker was 25 bp DNA step ladder, G451A. | ||
| + | |||
| + | The lanes were M (for the marker), 2 (plasmid), 3 (promoter) & 4 (RBS) | ||
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Results: We could visualize the biobrick parts. | Results: We could visualize the biobrick parts. | ||
| - | Transformation of E. coli using the part terminator, for which we followed the BioBrick protocol. | + | Transformation of '''E. coli''' using the part terminator, for which we followed the BioBrick protocol. |
{{:Team:Panama/calendar2}} | {{:Team:Panama/calendar2}} | ||
Revision as of 01:04, 15 October 2010
July 28
Electrophoresis of the digested parts:
4% 3:1 Agarose (Amresco, Cat no: 9012-36-6)
100ml TBE 1X.
4g of agarose
The agarose is disolved in the TBE using heat. At first, the mixture is opaque but as the agarose disolves, the mixture becomes clear. At this point we add 5ul of Ethydium bromide. This can then be poured into the casting chamber.
Agarose gel:
Once the gel was set, in each well we added 20 ul of sample, plus 2ul of loading buffer. Total volume = 22ul.
The molecular weight marker was 25 bp DNA step ladder, G451A.
The lanes were M (for the marker), 2 (plasmid), 3 (promoter) & 4 (RBS)
Results: We could visualize the biobrick parts.
Transformation of E. coli using the part terminator, for which we followed the BioBrick protocol.
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