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Team:Panama/26 July 2010
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| + | The reporter had problems during extraction. We obtained a small amount of plasmid, probably because we cultured it for more than 16 hours and we obtained low yield. | ||
We couldn’t visualize the expected bands, probably because in the first miniprep, we did not obtain enough DNA concentration. For this reason, we incubated more of the transformed E. coli in LB broth for 16h at 37°C. | We couldn’t visualize the expected bands, probably because in the first miniprep, we did not obtain enough DNA concentration. For this reason, we incubated more of the transformed E. coli in LB broth for 16h at 37°C. | ||
{{:Team:Panama/calendar2}} | {{:Team:Panama/calendar2}} | ||
Revision as of 00:51, 15 October 2010
July 26
We performed miniprep, for the E. coli transformed with the RBS, GFP and the promoter plasmid. And then we performed an extraction of the plasmid DNA.
DNA concentration was measured with the spectrometer. The measurements were performed with 260nm and 280nm, we obtained:
[RBS]=55,7 ηg/μl
[Promoter]= 7,18 μg/ml
For the analysis of the plasmid size, we prepared a 1% agarose gel.
Plasmids expected size:
RBS: psB1A2=2058bp, insert= 34bp; 2092bp
GFP: psB1A2=2058bp, insert= 742bp; 2800bp
Promoter: psB1A2=2058bp, insert= 45bp; 2103bp
The reporter had problems during extraction. We obtained a small amount of plasmid, probably because we cultured it for more than 16 hours and we obtained low yield. We couldn’t visualize the expected bands, probably because in the first miniprep, we did not obtain enough DNA concentration. For this reason, we incubated more of the transformed E. coli in LB broth for 16h at 37°C.
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