Team:Panama/21 October 2010

From 2010.igem.org

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(October 21)
(October 21)
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Calculations for Mutagenesis Primers
 
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1. 300 ug/ 334 ul water = 898 ng/ul
 
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1/10 dilution
 
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Take 1.11 ul of diluted stock for PCR
 
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2. 230 ug/279 ul water we have this concentration of primers diluted which is the same as 824 ng/ul
 
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Do 1/10 dilution (90 ul water and 10 ul of oligo
 
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Take 1.21 ul of diluted stock for PCR
 
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3. 280 ug/446 ul water = 600 ng/ul
 
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Dilute 1/10
 
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Take 1.67 ul of diluted stock for PCR
 
{{:Team:Panama/calendar2}}
{{:Team:Panama/calendar2}}

Revision as of 00:15, 28 October 2010

iGEM Panama

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October 21

Electrophoresis: Miniprep Samples 0.8% agarose, 1x TBE, 1.5 ul EtBr

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Sample 6 is a possible plasmid with insert

1) PCR sample 6

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2. Digest with EcoR1

Digestion of Sample 6 with EcoR1 Take 1.21 ul for 100 ng



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