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Team:Panama/21 October 2010
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0.8% agarose, 1x TBE, 1.5 ul EtBr | 0.8% agarose, 1x TBE, 1.5 ul EtBr | ||
| - | [[Image: | + | [[Image:Cuadro21octubre1.JPG|550px|thumb|left|alt text]] |
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| + | Sample 6 is a possible plasmid with insert | ||
1) PCR sample 6 | 1) PCR sample 6 | ||
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[[Image:UntitledPCR_sample6_Oct21.JPG|200px|thumb|left|alt text]] | [[Image:UntitledPCR_sample6_Oct21.JPG|200px|thumb|left|alt text]] | ||
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| - | 3. | + | |
| - | + | '''Results''' | |
| - | Take 1. | + | [[Image:Cuadro21octubre3.JPG|300px|thumb|left|alt text]] |
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| + | [[Image:UntitledOct21.JPG|200px|thumb|left|alt text]] | ||
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| + | We used to digest with Pstl the colony #3 obtained from quick screening on 20 October, as the band profiles obtained from our gene seemed possessed Rh1AB. | ||
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| + | 2. Digest with EcoR1 | ||
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| + | Digestion of Sample 6 with EcoR1 | ||
| + | Take 1.21 ul for 100 ng | ||
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{{:Team:Panama/calendar2}} | {{:Team:Panama/calendar2}} | ||
Latest revision as of 02:20, 28 October 2010
October 21
Electrophoresis: Miniprep Samples 0.8% agarose, 1x TBE, 1.5 ul EtBr
Sample 6 is a possible plasmid with insert
1) PCR sample 6
Results
We used to digest with Pstl the colony #3 obtained from quick screening on 20 October, as the band profiles obtained from our gene seemed possessed Rh1AB.
2. Digest with EcoR1
Digestion of Sample 6 with EcoR1 Take 1.21 ul for 100 ng
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