Team:Panama/20 October 2010
From 2010.igem.org
October 20
Digestion of pGEM-T + Rh1ab to compare with the non-digested and verify the difference in size Note: EcoR1 releases the insert.
Quick Check: Transformation of E. Coli with pGEM-T +Rh1ab
-100 ul culture -50 ul phenol:chloroform -10 ul loading buffer
Vortex
Electrophoresis: Quick Check 0.8% agarose, with 1x TBE, 1.5 ul EtBr Samples:4, 14, 13, 22, 8, 6, 16, 12, 9, 25, 5, 1 (C-), 15, 27, 28, 10, 3, 31, 29, 11
Homemade minipreps of Samples 1, 3, 5, 6, 10, 11, 12, 13, 16, 31
Cloning: pGEM-T + Rh1ab -Ligation: Overnight at 16C Ratio of insert:vector as 1:1
3 ul of PCR product
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